Query：
学者姓名：罗云敬
Refining：
Year
Type
Indexed by
Source
Complex
Co-Author
Language
Clean All
Abstract ：
为实现对稻米加工副产品营养物质的快速检测，按需进行饲料加工和加工副产品分级，研究选用302份稻米加工副产品样品（碎米107份、腹白米77份及异色粒118份），经过标准方法理化数据检测、样品前处理、近红外光谱采集、光谱数据预处理、筛选特征波长和建立模型等步骤，最终建立了针对碎米、腹白米及异色粒3种稻米加工副产品总体的水分、粗蛋白、粗脂肪以及粗纤维营养指标的总定量预测模型。结果显示：所建立的模型决定系数R~2均大于0.85，相对分析误差（RPD）均大于2.5，具有较好的准确性、稳定性。研究表明，此检测方法快速、高效、准确，提高了稻米加工副产品的综合利用率。
Keyword ：
粗蛋白 粗蛋白 碎米 碎米 异色粒 异色粒 粗脂肪 粗脂肪 腹白米 腹白米 粗纤维 粗纤维 水分 水分
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 翟晨 , 高曼 , 栾鑫鑫 et al. 稻米加工副产品营养指标的近红外预测模型的建立 [J]. | 饲料研究 , 2024 , (04) : 126-131 . |
| MLA | 翟晨 et al. "稻米加工副产品营养指标的近红外预测模型的建立" . | 饲料研究 04 (2024) : 126-131 . |
| APA | 翟晨 , 高曼 , 栾鑫鑫 , 钱承敬 , 张巍巍 , 史晓梅 et al. 稻米加工副产品营养指标的近红外预测模型的建立 . | 饲料研究 , 2024 , (04) , 126-131 . |
| Export to | NoteExpress RIS BibTex |
Abstract ：
单位点调控作为一种重要的材料修饰手段,近年来在催化、能源、环境等领域中蓬勃发展.调控单位点,可以有效地调控表面电荷、电子结构、原子空间构型,从而实现材料整体性能的提升.在拉曼检测领域,表面电荷等关键因素被广泛认可并是当下研究热点.然而,单位点对表面电荷调控,乃至对拉曼灵敏度影响尚无系统研究.该研究全新提出单位点(包括单分子、单原子、单原子中心的配体络合物等)的表面电荷调控作用并研究其对拉曼检测灵敏度的影响.其中,利用经典的特异性反应:4-氨基-3-肼基-5-巯基-1,2,4-三唑(AHMT)与甲醛生成6-巯基-5-三唑啉[4,3-b]-s-四嗪(MTT),使得经单位点AHMT调控的Ag材料具有极低的检测限10-12 mol·L-1,低于不经单位点AHMT调控表面电荷的10-9 mol·L-1,实现了对甲醛分子的超低浓度检测.还研究了单钨原子氧化物调控,对于非特异性反应中的标准分子、农药残留分子检测能力的影响.其中罗丹明6G的检测限可以从10-12 mol·L-1降低到10-14 mol·L-1,农药福美双的检测限也可以从10-9 mol·L-1降低到10-11 mol·L-1,据此提出了单位点调控表面电荷方法的普适性.此外,经过Zeta电位的测试,发现经单位点调控的Ag表面电位都有较大变化,符合库伦正负电荷吸附理论,这更有利于对待检测分子的捕捉,也是拉曼检测灵敏度提升的原因,同时也在机理上解释了单位点调控的普适性.对深层机理方面也做了许多猜测与研究:一方面,由于单位点与基底材料之间存在电荷转移,因而存在化学增强(CM)过程.同时,在Ag表面有显著的电磁场增强(EM),两种增强机制和单位点体系的协同,需要进一步通过实验、理论等,研究不同单位点的新物理机制;另一方面,单位点可能作为一种新的振动模态,与基底中存在的表面等离子体共振(SPR)、待测分子的共振协同作用,使得拉曼散射共振更强,从而提升拉曼检测灵敏度.该实验与结论都证实了单位点调控在拉曼检测中的可行性、必要性,使单位点在该领域的深入研究成为可能.
Keyword ：
单位点 单位点 表面电荷 表面电荷 均匀球状银颗粒 均匀球状银颗粒 普适性 普适性 定量化检测 定量化检测 表面增强拉曼散射 表面增强拉曼散射
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 孙志明 , 李辉 , 冯奕博 et al. 单位点表面电荷调控提升拉曼检测灵敏度 [J]. | 光谱学与光谱分析 , 2023 , 43 (4) : 1075-1082 . |
| MLA | 孙志明 et al. "单位点表面电荷调控提升拉曼检测灵敏度" . | 光谱学与光谱分析 43 . 4 (2023) : 1075-1082 . |
| APA | 孙志明 , 李辉 , 冯奕博 , 高钰航 , 裴佳欢 , 常莉 et al. 单位点表面电荷调控提升拉曼检测灵敏度 . | 光谱学与光谱分析 , 2023 , 43 (4) , 1075-1082 . |
| Export to | NoteExpress RIS BibTex |
Abstract ：
Gold nanoclusters have good biocompatibility and can be easily modified to improve their luminescence properties. In this study, we prepared a new type of dual-emitting gold nanoclusters (d-Au NCs) for discriminative detection of phenylalanine and Fe3+ with high selectivity and sensitivity. The fluorescence sensor which was synthesized without any further assembly or conjugation shows dual-emissions at 430 nm and 600 nm under a single excitation at 350 nm. Phenylalanine can turn on the red emission of the probe, while Fe3+ can turn on its yellow emission and turn off the red emission. By detecting a variety of amino acids and metal ions, d-Au NCs showed good selectivity to phenylalanine and Fe3+. Finally, this method was applied to determine phenylalanine and Fe3+ in lake water, human urine and milk, which has certain application prospects in the field of biology and environment.
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Kong, Chenchen , Luo, Yunjing , Zhang, Wei et al. A ratio fluorescence method based on dual emissive gold nanoclusters for detection of biomolecules and metal ions [J]. | RSC ADVANCES , 2022 , 12 (19) : 12060-12067 . |
| MLA | Kong, Chenchen et al. "A ratio fluorescence method based on dual emissive gold nanoclusters for detection of biomolecules and metal ions" . | RSC ADVANCES 12 . 19 (2022) : 12060-12067 . |
| APA | Kong, Chenchen , Luo, Yunjing , Zhang, Wei , Lin, Taifeng , Na, Zhen , Liu, Xuemei et al. A ratio fluorescence method based on dual emissive gold nanoclusters for detection of biomolecules and metal ions . | RSC ADVANCES , 2022 , 12 (19) , 12060-12067 . |
| Export to | NoteExpress RIS BibTex |
Abstract ：
Based on gold nanoclusters (AuNCs) with good optical stability, biocompatibility and simple and non-toxic preparation method, this paper developed a highly selective, highly sensitive and visualized uric acid (UA) sensor. We synthesized BSA-AuNCs using bovine serum albumin (BSA) as a template. Under the catalysis of urate oxidase, UA produces stoichiometric hydrogen peroxide (H2O2), which causes the fluorescence of AuNCs to be quenched. In addition, we found that BSA-AuNCs mimic the peroxidase activity in this system, catalysing the substrate's oxidation 3,3',5,5'-tetramethylbenzidine (TMB) to ox-TMB by H2O2. At this time, the emission spectrum of BSA-AuNCs overlaps the absorption spectrum of ox-TMB, and a kind of fluorescence resonance energy transfer (FRET) occurs. BSA-AuNCs acts as a donor to transfer the excitation energy to the acceptor ox-TMB, which makes ox-TMB produce fluorescence. At the same time, the fluorescence intensity of BSA-AuNCs is significantly lower than when it exists alone, which significantly improves the sensitivity of UA detection. Under optimum conditions, it is found that the UA concentration has an excellent linear relationship between the quenching degree of 2 similar to 100 mu mol . L-1, the linear equation is (F-0 - F)/F-0=0. 005 85c(UA) + 0. 103 64, the linear correlation coefficient is 0. 995 4, and the detection limit is 0. 26 mu mol . L-1, which is far below the minimum limit of normal human UA levels (90 mu mol . L-1). Meanwhile, the recovery of UA in blood samples was studied, and the recovery rate was 97. 3% to 104. 7%, indicating that this method is effective in clinical blood samples. The detection has tremendous application potential, and provides an excellent theoretical basis and methodological guidance for further clinical analysis.
Keyword ：
Hydrogen peroxide Hydrogen peroxide Fluorescence resonance energy transfer Fluorescence resonance energy transfer Gold nanoclusters Gold nanoclusters Uric acid Uric acid
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Cong Jian-han , Luo Yun-jing , Qi Xiao-hua et al. Sensitive Detection of Uric Acid Based on BSA Gold Nanoclusters by Fluorescence Energy Resonance Transfer [J]. | SPECTROSCOPY AND SPECTRAL ANALYSIS , 2022 , 42 (2) : 483-489 . |
| MLA | Cong Jian-han et al. "Sensitive Detection of Uric Acid Based on BSA Gold Nanoclusters by Fluorescence Energy Resonance Transfer" . | SPECTROSCOPY AND SPECTRAL ANALYSIS 42 . 2 (2022) : 483-489 . |
| APA | Cong Jian-han , Luo Yun-jing , Qi Xiao-hua , Zou Ming-qiang , Kong Chen-chen . Sensitive Detection of Uric Acid Based on BSA Gold Nanoclusters by Fluorescence Energy Resonance Transfer . | SPECTROSCOPY AND SPECTRAL ANALYSIS , 2022 , 42 (2) , 483-489 . |
| Export to | NoteExpress RIS BibTex |
Abstract ：
In this study, morin magnetic molecularly imprinted polymers (Morin-MMIPs) were synthesized based on magnetic nanoparticles and surface molecularly imprinted technology with superparamagnetism and extraction selectivity. The polymers allowed the separating of morin from complex matrices in the presence of an external magnetic field with no need for centrifugation or filtration. The microstructure of the polymers was characterized by scanning electron microscopy and transmission electron microscopy. Meanwhile, the functional group and magnetic properties of the polymers were characterized using Fourier transform infrared spectroscopy (FT-IR) and magnetic vibration meter (VSM). The maximum adsorption capacity of MMIPs was 3.24 mg/g, which was 2.55 times higher than that of MNIPs (1.27 mg/g). Morin was quantified by HPLC-DAD, which showed good linearity in the concentration range of 0.05-60 mu g/ml with the correlation coefficient R-2 = 0.9993. The limit of detection (LOD) was 0.08 mu g/ml, and the spiked recoveries were 87.5-106.8%. The calculation of the adsorption isotherm and kinetic model revealed the adsorption mechanisms, and the adsorption process was consistent with the Langmuir adsorption isotherm and pseudo-secondary kinetic models. Likewise, the material has been successfully used to extract and separate morin from food samples. The method reported in this paper has the advantages of fast adsorption speed, high selectivity, and environmental friendliness. It provided a reliable method for the separation and detection of morin or other natural products.
Keyword ：
magnetic molecularly imprinted polymers magnetic molecularly imprinted polymers molecular imprinting technology molecular imprinting technology selective extraction selective extraction morin morin
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Zhang, Wei , Luo, Yunjing , Xie, Ziqi et al. Extraction and detection of morin from Sanghuangporus lonicericola by magnetic molecularly imprinted polymers coupled with HPLC analysis [J]. | JOURNAL OF FOOD SCIENCE , 2022 , 87 (4) : 1575-1585 . |
| MLA | Zhang, Wei et al. "Extraction and detection of morin from Sanghuangporus lonicericola by magnetic molecularly imprinted polymers coupled with HPLC analysis" . | JOURNAL OF FOOD SCIENCE 87 . 4 (2022) : 1575-1585 . |
| APA | Zhang, Wei , Luo, Yunjing , Xie, Ziqi , Kong, Chenchen , Na, Zhen . Extraction and detection of morin from Sanghuangporus lonicericola by magnetic molecularly imprinted polymers coupled with HPLC analysis . | JOURNAL OF FOOD SCIENCE , 2022 , 87 (4) , 1575-1585 . |
| Export to | NoteExpress RIS BibTex |
Abstract ：
目的 建立QuEChERS-超高效液相色谱-三重四极杆质谱法快速检测主要原粮与油料中101种常见农药残留的分析方法.方法 样品加入乙腈后涡旋混匀并超声提取,采用无水硫酸镁和N-丙基硅烷化硅胶(primary secondary amine,PSA)固相吸附剂净化,以乙腈-0.1％甲酸水溶液(V:V)作为流动相进行梯度洗脱,采用T3色谱柱(100 mm×2.1 mm,1.7μm)分离,在多反应监测(multiple reaction monitoring,MRM)模式下进行测定,外标法定量.结果 101种农药残留在3个添加水平(50、100和200μg/kg)下回收率在62.31％～119.74％之间,相对标准偏差小于15％,定量限为0.06～23.81μg/kg.结论 该方法操作简便、快速,有较高的灵敏度和准确度,可应用于主要原粮与油料中农药残留日常筛查工作.
Keyword ：
QuEChERS QuEChERS 主要原粮与油料 主要原粮与油料 超高效液相色谱-三重四极杆质谱法 超高效液相色谱-三重四极杆质谱法 农药残留 农药残留
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 王柄钧 , 史晓梅 , 张巍巍 et al. QuEChERS-超高效液相色谱-三重四极杆质谱法快速检测主要原粮与油料中101种农药残留 [J]. | 食品安全质量检测学报 , 2021 , 12 (18) : 7272-7280 . |
| MLA | 王柄钧 et al. "QuEChERS-超高效液相色谱-三重四极杆质谱法快速检测主要原粮与油料中101种农药残留" . | 食品安全质量检测学报 12 . 18 (2021) : 7272-7280 . |
| APA | 王柄钧 , 史晓梅 , 张巍巍 , 张江旭 , 赵文君 , 罗云敬 et al. QuEChERS-超高效液相色谱-三重四极杆质谱法快速检测主要原粮与油料中101种农药残留 . | 食品安全质量检测学报 , 2021 , 12 (18) , 7272-7280 . |
| Export to | NoteExpress RIS BibTex |
Abstract ：
热休克蛋白90B1又称为糖蛋白96 (gp96). gp96属于热休克蛋白90家族,是一种高度保守且普遍存在的糖蛋白.作为一种内质网蛋白,gp96在维持内质网稳态、内质网应激、钙稳态等方面起着重要的调控作用,这些调控网络在肿瘤的发生发展过程中起着重要的作用. gp96作为分子伴侣在稳定和激活客户蛋白等方面有众多报道,其中包括HER2、整合素和Toll样受体等多个客户蛋白.大量研究表明,gp96在肝癌、乳腺癌、胃癌等不同类型的肿瘤中高表达,并在肿瘤的生长、侵袭和转移等方面起着重要的作用.本文从gp96的基本结构和功能及其在肿瘤发生发展中的作用等方面进行综述,并着重阐述细胞膜gp96相关的研究进展...
Keyword ：
热休克蛋白gp96 热休克蛋白gp96 靶向治疗 靶向治疗 肿瘤 肿瘤 分子伴侣 分子伴侣 胞膜gp96 胞膜gp96
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 钱丽媛 , 李长菲 , 罗云敬 et al. 热休克蛋白gp96在恶性肿瘤中的研究进展 [J]. | 生物化学与生物物理进展 , 2021 , 48 (09) : 993-1005 . |
| MLA | 钱丽媛 et al. "热休克蛋白gp96在恶性肿瘤中的研究进展" . | 生物化学与生物物理进展 48 . 09 (2021) : 993-1005 . |
| APA | 钱丽媛 , 李长菲 , 罗云敬 , 孟颂东 . 热休克蛋白gp96在恶性肿瘤中的研究进展 . | 生物化学与生物物理进展 , 2021 , 48 (09) , 993-1005 . |
| Export to | NoteExpress RIS BibTex |
Abstract ：
肝细胞癌(Hepatocellular carcinoma,HCC)是致死率第3的恶性肿瘤,也是全球第5大常见癌症。肝癌在临床上的治疗手段非常有限,患者的总生存率也很低。因此,肝癌的早期诊断和治疗对于患者总生存率有着重要的影响。甲胎蛋白(Alpha-fetoprotein,AFP)是最早发现也是目前应用最广泛的肝癌标志物之一。目前,多项研究表明,作为一个特异性的癌基因,AFP在肝癌的发生、发展、诊断和治疗中有巨大的研究价值。文中简述了AFP在肝癌发生发展中的分子调控机制以及在肝癌细胞逃避免疫监视中的作用,着重阐述AFP作为重要的肝癌靶标分子在肝癌的临床诊断和治疗研究中的应用。
Keyword ：
甲胎蛋白 甲胎蛋白 肝癌治疗 肝癌治疗 肝癌 肝癌 临床诊断 临床诊断 血清学标志物 血清学标志物
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 钱丽媛 , 李长菲 , 罗云敬 et al. 甲胎蛋白在肝癌的诊断和治疗中的研究进展 [J]. | 生物工程学报 , 2021 , 37 (09) : 3042-3060 . |
| MLA | 钱丽媛 et al. "甲胎蛋白在肝癌的诊断和治疗中的研究进展" . | 生物工程学报 37 . 09 (2021) : 3042-3060 . |
| APA | 钱丽媛 , 李长菲 , 罗云敬 , 孟颂东 . 甲胎蛋白在肝癌的诊断和治疗中的研究进展 . | 生物工程学报 , 2021 , 37 (09) , 3042-3060 . |
| Export to | NoteExpress RIS BibTex |
Abstract ：
Glycoprotein 96 (gp96) is a highly conserved and ubiquitous glycoprotein that belongs to the heat shock protein 90 (HSP90) family. It comprises 4 domains: N-terminal domain (NTD), middle domain (MD), C-terminal domain (CTD) and charged linker region (CR). Each domain performs a specific function. NTD containing the nucleotide binding site interacts with and hydrolyzes ATP. MD is involved in client protein recognition, and is the site of constitutive dimerization. The ATP hydrolytic activity of NTD requires cooperative action of CR and MD. The schematic representation of gp96 topology is shown in the main text. An increased expression of gp96 has been reported in multiple cancers. Its upregulation in tumors is closely correlated with poor prognosis and decreased overall survival of patients, indicating that gp96 serves as a potential diagnostic and prognostic biomarker. As a chaperone protein gp96 directs the folding and/or assembly of secreted and membrane proteins. It has a limited client protein profile that is involved in key processes linked with the hallmarks of cancer. Previous studies have shown that cellular gp96 physically interacts with and directs the folding and assembly of several client proteins, including insulin-like growth factors (IGF), integrins, epidermal growth factor receptor-2 (HER2) and Wnt co-receptor LRP6, which are involved in the regulation of cell multiplication, normal tissue differentiation, cancer progression and metastasis. It has been found that gp96 was only expressed on cell surface of malignant tumor but not benign tissues. Cell membrane gp96 is closely associated with cancer cell proliferation, invasion, and metastasis. We further demonstrated that gp96 on cell membrane interacts with HER2, urokinase-type plasminogen activator-receptor (uPAR) or ER-alpha 36. Targeting gp96 by siRNA or a monoclonal antibody for gp96 led to decreased cell growth and invasion, increased apoptosis in vitro, and suppression of tumor growth in vivo, validating cell membrane gp96 as a therapeutic target. At present, the selective small-molecule inhibitors (NECA and PU-WS13), a gp96-specific monoclonal antibody (W9mAb), and an inhibitory gp96-targeted polypeptide (p37) are under development and their potential applications in the tumor targeting therapy are highlighted in this review.
Keyword ：
heat shock protein gp96 heat shock protein gp96 molecular chaperone molecular chaperone targeted therapy targeted therapy cancer cancer cell membrane gp96 cell membrane gp96
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Qian Li-Yuan , Li Chang-Fei , Luo Yun-Jing et al. Research Progress of The Role of Heat Shock Protein gp96 in Cancer Development and Progression [J]. | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS , 2021 , 48 (9) : 993-1005 . |
| MLA | Qian Li-Yuan et al. "Research Progress of The Role of Heat Shock Protein gp96 in Cancer Development and Progression" . | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 48 . 9 (2021) : 993-1005 . |
| APA | Qian Li-Yuan , Li Chang-Fei , Luo Yun-Jing , Meng Song-Dong . Research Progress of The Role of Heat Shock Protein gp96 in Cancer Development and Progression . | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS , 2021 , 48 (9) , 993-1005 . |
| Export to | NoteExpress RIS BibTex |
Abstract ：
目的:建立QuEChERS-气相色谱-串联质谱(GC-MS/MS)快速测定谷类中179种农药残留的方法,并应用于191份谷类样品。方法:样品经乙腈提取,N-丙基乙二胺(PSA)和十八烷基硅烷键合硅胶(C_(18))净化,气相色谱-串联质谱多反应离子监测(MRM)模式检测。结果:179种农药在质量浓度为0.02～0.5 mg/L范围内线性关系良好,相关系数均大于0.996,检出限和定量限分别为0.001～0.016 mg/kg和0.003～0.050 mg/kg,在0.025 mg/L、0.050 mg/L和0.100 mg/L添加水平下,农药的回收率为63.7%～117.2%,相对标准偏差(R...
Keyword ：
气相色谱-串联质谱法 气相色谱-串联质谱法 谷类 谷类 测定 测定 农药残留 农药残留 QuEChERS QuEChERS
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 席甲甲 , 张巍巍 , 王柄钧 et al. 气相色谱-串联质谱法快速测定谷类中179种农药残留 [J]. | 食品安全导刊 , 2021 , PageCount-页数: 3 (18) : 137-139 . |
| MLA | 席甲甲 et al. "气相色谱-串联质谱法快速测定谷类中179种农药残留" . | 食品安全导刊 PageCount-页数: 3 . 18 (2021) : 137-139 . |
| APA | 席甲甲 , 张巍巍 , 王柄钧 , 史晓梅 , 罗云敬 , 钱承敬 . 气相色谱-串联质谱法快速测定谷类中179种农药残留 . | 食品安全导刊 , 2021 , PageCount-页数: 3 (18) , 137-139 . |
| Export to | NoteExpress RIS BibTex |
Export
| Results： |
Selected to |
| Format： |
