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学者姓名：彭永臻
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Abstract ：
为探究低C/N城镇污水处理在无外加碳源的情况下实现深度脱氮除磷的效能，试验以实际城镇生活污水为处理对象，搭建了日处理规模为100 m~3·d~(-1)的A~2/O-BCO前置反硝化除磷中试，并从中试快速启动、工况调整与优化、微生物群落分析等角度进行研究.结果表明，通过先在A~2/O单元与BCO单元中分别富集聚磷菌与硝化菌，再增加硝化液回流以富集反硝化除磷菌（DPAOs）的方式，可实现A~2/O-BCO前置反硝化除磷中试的快速启动.A~2/O单元厌氧/缺氧/好氧容积比调整为3∶5∶1，硝化液回流比设定为250%时，缺氧段对磷去除率的平均值达到95%，平均出水PO_4~(3-)-P浓度小于0.2 mg·L~(-1)，出水TN小于11 mg·L~(-1).通过分子生物学分析发现，除磷菌（PAOs）与硝化菌丰度显著提高，Candidatus Accumulibacter的相对丰度从原始种泥的2%增加到14%，已被证实具有反硝化聚磷功能的Dechloromonas的相对丰度从原始种泥的2%增加到7%,BCO单元中的生物膜上，Nitrosomonas的相对丰度为5.91%,Nitrospira的相对丰度为7.58%.
Keyword ：
前置反硝化除磷 前置反硝化除磷 快速启动 快速启动 无外加碳源 无外加碳源 低C/N城镇污水 低C/N城镇污水 中试规模 中试规模
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| GB/T 7714 | 周庆国 , 苏健 , 陈东旭 et al. A~2/O-BCO前置反硝化除磷中试的启动与优化运行 [J]. | 环境科学学报 , 2024 , 44 (03) : 13-22 . |
| MLA | 周庆国 et al. "A~2/O-BCO前置反硝化除磷中试的启动与优化运行" . | 环境科学学报 44 . 03 (2024) : 13-22 . |
| APA | 周庆国 , 苏健 , 陈东旭 , 彭永臻 . A~2/O-BCO前置反硝化除磷中试的启动与优化运行 . | 环境科学学报 , 2024 , 44 (03) , 13-22 . |
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Abstract ：
Challenges in obtaining stable nitrite have impeded the use of anammox in municipal wastewater treatment. This study explored the feasibility of using sludge fermentation products as carbon source and selective nitrification inhibitor to supply nitrite via partial nitrification (PN) and partial denitrification (PD). PD was initiated within 15 days, achieving nitrite transformation rate of over 90 % with a carbon/nitrogen ratio of 3 and a reaction time of 0.75 h. The dominant genus, Romboutsia, increased in relative abundance from 4.1 to 35 %. Organic acids in sludge fermentation products, like acetate (200 mg/L) and propionate (400 mg/L), selectively suppressed nitriteoxidizing bacteria (NOB) more than ammonia-oxidizing bacteria (AOB), leading to PN. Combining anaerobic exposure with sludge fermentation products addition achieved PN with over 80.0 % nitrite accumulation. AOB increased tenfold in the long term, significantly outpacing NOB growth. This strategy simplifies difficulty of anammox application and shows broad application potential in municipal wastewater treatment.
Keyword ：
Short-chain fatty acids Short-chain fatty acids Carbon source Carbon source Microbial community Microbial community Nitrification inhibitor Nitrification inhibitor Nitrite accumulation Nitrite accumulation
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| GB/T 7714 | Liu, Jinjin , Wang, Rui , Qiu, Shengjie et al. Feasibility of double nitrite supply through partial nitrification and partial denitrification driven by sludge fermentation [J]. | BIORESOURCE TECHNOLOGY , 2024 , 414 . |
| MLA | Liu, Jinjin et al. "Feasibility of double nitrite supply through partial nitrification and partial denitrification driven by sludge fermentation" . | BIORESOURCE TECHNOLOGY 414 (2024) . |
| APA | Liu, Jinjin , Wang, Rui , Qiu, Shengjie , Peng, Yi , Peng, Yongzhen . Feasibility of double nitrite supply through partial nitrification and partial denitrification driven by sludge fermentation . | BIORESOURCE TECHNOLOGY , 2024 , 414 . |
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Abstract ：
Anammox-based processes are pivotal for elevating nitrogen removal efficiency in municipal wastewater treatment. This study established a novel HF-EPDA system combined in-situ hydrolytic fermentation (HF) with endogenous partial denitrification (EPD) and anammox. Slowly-biodegradable organic matter (SBOM) was degraded and transformed into endogenous polymers for driving production of sufficient nitrite by EPD, further promoted the nitrogen removal via anammox process. Processes above formed positive feedback, guaranteeing the robustness and recoverability of system. After a 92-day suspension during operation, advanced nitrogen removal was still achieved with excellent nitrogen removal efficiency of 95.84 +/- 1.73 %, treating with actual domestic wastewater and synthetic nitrate wastewater. Candidatus Brocadia and Candidatus Competibacter were dominant bacteria on biofilms responsible for the anammox and EPD process respectively, while the main hydrolytic fermentation organisms norank_o SBR1031 was enriched in floc sludge. This study highlights the reliable potential for expanding anammox application with simultaneous improvement of SBOM utilization.
Keyword ：
Slowly-biodegradable organic matter (SBOM) Slowly-biodegradable organic matter (SBOM) Anammox Anammox Hydrolytic fermentation (HF) Hydrolytic fermentation (HF) Endogenous partial denitrification (EPD) Endogenous partial denitrification (EPD)
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| GB/T 7714 | Xie, Chen , Li, Xiyao , Zhang, Qiong et al. Achieving advanced nitrogen removal with anammox and endogenous partial denitrification driven by efficient hydrolytic fermentation of slowly-biodegradable organic matter [J]. | BIORESOURCE TECHNOLOGY , 2024 , 414 . |
| MLA | Xie, Chen et al. "Achieving advanced nitrogen removal with anammox and endogenous partial denitrification driven by efficient hydrolytic fermentation of slowly-biodegradable organic matter" . | BIORESOURCE TECHNOLOGY 414 (2024) . |
| APA | Xie, Chen , Li, Xiyao , Zhang, Qiong , Zhang, Lujing , Cao, Xiaoxin , Peng, Yongzhen . Achieving advanced nitrogen removal with anammox and endogenous partial denitrification driven by efficient hydrolytic fermentation of slowly-biodegradable organic matter . | BIORESOURCE TECHNOLOGY , 2024 , 414 . |
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Abstract ：
Anaerobic ammonia oxidation (Anammox) exhibits promise for wastewater treatment, but the enrichment of anammox bacteria (AnAOB) in municipal wastewater treatment plants is a significant challenge. This study constructed a novel Anoxic-Anaerobic-Oxic An aerobic-Oxic (AAnO) process with a pure biofilm anoxic zone fed with actual fluctuating municipal wastewater and operated for six months to enrich AnAOB at ambient temperature. High- throughput sequencing (HTS), qPCR, and fluorescence in situ hybridization showed that AnAOB were successfully enriched in the anoxic biofilms, reaching 1.56 % relative abundance on day 75 detected by HTS. During the period from day 130 to day 186, the anammox process contributed to 55.8 +/- 19.2 % of the nitrogen removal in the anoxic zone. Phylogenetic analysis revealed this AnAOB species was closely related to Candidatus Brocadia fulgida. . This study provides technical support for the application of anammox in mainstream wastewater.
Keyword ：
Biofilm Biofilm Partial denitrification Partial denitrification Fluctuating municipal wastewater Fluctuating municipal wastewater Ambient temperature Ambient temperature
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| GB/T 7714 | Chen, Guo , Li, Jianwei , Zhang, Shujun et al. Novel Anoxic-Anaerobic-Oxic process successfully enriched anammox bacteria under actual municipal wastewater [J]. | BIORESOURCE TECHNOLOGY , 2024 , 412 . |
| MLA | Chen, Guo et al. "Novel Anoxic-Anaerobic-Oxic process successfully enriched anammox bacteria under actual municipal wastewater" . | BIORESOURCE TECHNOLOGY 412 (2024) . |
| APA | Chen, Guo , Li, Jianwei , Zhang, Shujun , Gao, Xiaoyu , Gu, Changkun , Lv, Xintao et al. Novel Anoxic-Anaerobic-Oxic process successfully enriched anammox bacteria under actual municipal wastewater . | BIORESOURCE TECHNOLOGY , 2024 , 412 . |
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Abstract ：
For low carbon-to-nitrogen ratio municipal wastewater, full utilization of carbon sources is positive in reducing carbon emissions and carbon source addition. This study evaluated and proposed appropriate anaerobic duration control to improve nitrogen and phosphorus removal performance in Denitrifying Phosphorus Removal and Partial Denitrification coupled with Anammox (DPR-PDA) system. Excessive anaerobic duration resulted in wasted carbon source, anaerobic stage should be stopped after carbon source had been utilized. Hydraulic Retention Time-Anaerobic (HRT-An) of R1 was shortened from 5h to 0.56h, while R2 was maintained at 5h. The removal efficiency of nitrate (NO3-) were 91.7% in R1 and 71.5% in R2. Peak of NO2- accumulation (Peak-NO2--N) during denitrification in R1 was 4.5 times than in R2. Candidatus_Brocadia as anammox bacteria was self-enriched, the abundance in R1 was 4.67 times than in R2. This provided a practical guide for optimization of wastewater treatment processes and application of anammox technology.
Keyword ：
Anammox bacteria self-enrichment Anammox bacteria self-enrichment Anaerobic duration Anaerobic duration Nitrite accumulation Nitrite accumulation Carbon source utilization Carbon source utilization
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| GB/T 7714 | Su, Yunlong , Li, Xiyao , Wang, Jiao et al. Effect of anaerobic duration on nitrogen and phosphorus removal and carbon source utilization in integrated denitrifying phosphorus removal and partial denitrification coupled with anammox system [J]. | JOURNAL OF CLEANER PRODUCTION , 2024 , 474 . |
| MLA | Su, Yunlong et al. "Effect of anaerobic duration on nitrogen and phosphorus removal and carbon source utilization in integrated denitrifying phosphorus removal and partial denitrification coupled with anammox system" . | JOURNAL OF CLEANER PRODUCTION 474 (2024) . |
| APA | Su, Yunlong , Li, Xiyao , Wang, Jiao , Du, Rui , Xue, Xiaofei , Shi, Yanwei et al. Effect of anaerobic duration on nitrogen and phosphorus removal and carbon source utilization in integrated denitrifying phosphorus removal and partial denitrification coupled with anammox system . | JOURNAL OF CLEANER PRODUCTION , 2024 , 474 . |
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Abstract ：
Achieving stable and high-rate partial nitrification (PN) remains a worldwide technical conundrum in lowstrength mainstream conditions. This study successfully achieved ultrarapid mainstream PN within 8 days under a saturated dissolved oxygen (DO) supply strategy, reaching a record-breaking PN rate of over 1.0 kg N m(- 3) d(- 1) treating municipal wastewater. Stable PN was maintained for over 200 days with an ultrahigh nitrite accumulation ratio of 98.5 +/- 0.9 %, resilient to seasonal fluctuations in temperature (16.0-25.6 degrees C) and load (NH4+-N, 40-80 mg N/L). Kinetics revealed a remarkable 159.1-fold increase in the maximum activity ratio of ammonia-oxidizing bacteria (AOB) to nitrite-oxidizing bacteria (NOB). The faster response of AOB to saturated DO stimulated its highest activity difference with NOB, contributing to the AOB (Nitrosomonas oligotropha) boom and the elimination of NOB groups (-99.9 %). Our results highlight the importance of promoting AOB rather than solely focusing on NOB suppression for initiating and stabilizing high-rate mainstream PN.
Keyword ：
Dissolved oxygen Dissolved oxygen Mainstream wastewater Mainstream wastewater NOB elimination NOB elimination Nitrite accumulation Nitrite accumulation Ammonium oxidation rate Ammonium oxidation rate
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| GB/T 7714 | Dan, Qiongpeng , Li, Xiyao , Zhang, Fangzhai et al. Saturated dissolved oxygen-driven high-rate and ultrastable partial nitrification in municipal wastewater [J]. | BIORESOURCE TECHNOLOGY , 2024 , 413 . |
| MLA | Dan, Qiongpeng et al. "Saturated dissolved oxygen-driven high-rate and ultrastable partial nitrification in municipal wastewater" . | BIORESOURCE TECHNOLOGY 413 (2024) . |
| APA | Dan, Qiongpeng , Li, Xiyao , Zhang, Fangzhai , Du, Rui , Li, Jialin , Wang, Tong et al. Saturated dissolved oxygen-driven high-rate and ultrastable partial nitrification in municipal wastewater . | BIORESOURCE TECHNOLOGY , 2024 , 413 . |
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Abstract ：
为应对严重的水污染问题，污水排放标准越来越严格。污染物的深度去除，通常需要消耗大量的电能并经常性投加大量的化学药剂，这导致污水厂间接碳排放量巨大，环境效益降低，违背低碳可持续发展的原则。污水碳中和的达成可通过节能降耗、资源回收以及自身产能来实现。在能源紧缺，实现碳中和迫在眉睫的背景下，全面推进节能降耗技术的应用是最现实且有望短期实现的策略。脱氮是目前城市污水处理厂的关键任务，能耗占比大，本文即针对现有的污水脱氮途径和技术，从降低能源消耗以及实现资源回收两个方面出发，对能耗低、具有可持续性且具有应用前景的工艺进行总结。重点对厌氧氨氧化工艺（Anaerobic ammonium oxidation, Anammox），以及能实现能源回收的CANDO(Coupled aerobic-anoxic nitrous decomposition operation)工艺进行更为全面系统的综述，指出现有的技术问题和研究瓶颈，以期为污水脱氮技术的低碳发展及工程应用提供参考。
Keyword ：
污水 污水 可持续 可持续 碳中和 碳中和 厌氧氨氧化 厌氧氨氧化 脱氮 脱氮
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| GB/T 7714 | 王伟 , 王明刚 , 刘丽娜 et al. 面向碳中和的污水低能耗脱氮及氮回收技术探讨 [J]. | 水处理技术 , 2023 , 49 (04) : 1-6 . |
| MLA | 王伟 et al. "面向碳中和的污水低能耗脱氮及氮回收技术探讨" . | 水处理技术 49 . 04 (2023) : 1-6 . |
| APA | 王伟 , 王明刚 , 刘丽娜 , 张鑫 , 彭永臻 . 面向碳中和的污水低能耗脱氮及氮回收技术探讨 . | 水处理技术 , 2023 , 49 (04) , 1-6 . |
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Abstract ：
Anaerobic ammonia oxidation bacteria (AnAOB) are difficult to cultivate due to their long start-up time and sensitivity to environmental conditions. In this study, anammox granular sludge was cultured with ordinary activated sludge under influent dissolved oxygen concentrations of 6-8 mg/L, successfully enriching AnAOB. The presence of multiple microorganisms in the activated sludge enabled the anammox system to resist the unfavorable influent environment and sustain system stability. The total nitrogen removal rate reached a maximum of 81%, and the TN effective load increased from 0.1 to 1.5 kg N/m(3)/d. The results showed that the dissolved oxygen present in the influent did not lead to a breakdown in the anammox system. The protein in the sludge extracellular polymeric substances played an important role in the enrichment of AnAOB, and the sludge settling performance at the bottom of the reactor was better than that at the top of the reactor, with protein/polysaccharide in the range of 5-6.3. Candidatus brocadia and Candidatus kuenenia were the main anammox functional bacteria in the system. On 153 d of reactor operation, their relative abundances were 8.51 and 5.68%, respectively. This study shows that microorganisms in activated sludge contribute to the stability of the anammox system when the influent conditions are appropriate. This provides a new idea for the rapid start-up of the anammox system and enrichment of AnAOB.
Keyword ：
anammox anammox extracellular polymeric substances extracellular polymeric substances activated sludge activated sludge microbial communities microbial communities granular sludge granular sludge nitrogen removal nitrogen removal
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| GB/T 7714 | Liu, Lifang , Hu, Meiling , Wang, Cong et al. Enrichment of Anammox Bacteria Using Anammox Sludge as a Primer Combined with Ordinary Activated Sludge [J]. | SUSTAINABILITY , 2023 , 15 (16) . |
| MLA | Liu, Lifang et al. "Enrichment of Anammox Bacteria Using Anammox Sludge as a Primer Combined with Ordinary Activated Sludge" . | SUSTAINABILITY 15 . 16 (2023) . |
| APA | Liu, Lifang , Hu, Meiling , Wang, Cong , Qi, Weikang , Peng, Yongzhen . Enrichment of Anammox Bacteria Using Anammox Sludge as a Primer Combined with Ordinary Activated Sludge . | SUSTAINABILITY , 2023 , 15 (16) . |
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Abstract ：
In the gas biodesulfurization process, converting sulfides into elemental sulfur (S0) for reuse holds significant environmental, economic, resource, and social values. While the influence of pH on microbial community structure and enzyme activity is recognized, its effect on S0 yield has been underestimated. This study delves into how pH fluctuations affect the S0 yield in a biodesulfurization system. Under extremely acidic condition (pH < 1), the selectivity of S0 reached 90% even at high O2/H2S supply ratio of 1.8-2.1. Metagenomic analyses showed that as the pH decreased from neutral-alkaline to extremely acidic range, the dominant bacterium gradually shifted from Acidithiobacillus (37.0%), Acidiphilium (1.5%) and Thiomonas (1.2%), which possess complete sulfur metabolism (from sulfide to sulfate) genes, to Mycobacterium (79.2%) possessing the S0-synthesis gene (sqr) without the S0-oxidizing genes (ETHE1, sor, hdr, dsr and the Sox and S4I complex genes), resulting in a robust S0 yield in extremely acidic period. Various pathways for respiratory sulfur oxidation were present in different pH periods. The extremely acidic condition facilitated the selection of specific microorganisms by creating a highly selective environment, promoting the growth of acidophilic or acid-tolerant microorganisms with unique traits and altering the sulfur-metabolic pathways. These finding filled a knowledge gap regarding the impact of pH on sulfur-metabolic pathways and offered a new strategy for gaining S0.
Keyword ：
S0 recovery S0 recovery S0-producing bacterium S0-producing bacterium Sulfur-metabolic pathways Sulfur-metabolic pathways pH variation pH variation
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| GB/T 7714 | Jia, Tipei , Zhang, Liang , Peng, Yi et al. The enrichment of a specialized elemental sulfur-producing bacterium under extremely acidic condition [J]. | CHEMICAL ENGINEERING JOURNAL , 2023 , 472 . |
| MLA | Jia, Tipei et al. "The enrichment of a specialized elemental sulfur-producing bacterium under extremely acidic condition" . | CHEMICAL ENGINEERING JOURNAL 472 (2023) . |
| APA | Jia, Tipei , Zhang, Liang , Peng, Yi , Sun, Shihao , Li, Xiyao , Zhao, Qi et al. The enrichment of a specialized elemental sulfur-producing bacterium under extremely acidic condition . | CHEMICAL ENGINEERING JOURNAL , 2023 , 472 . |
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Abstract ：
The initiating and stable preservation of partial nitrification (PN) and achievement of anammox bacteria selfenrichment in domestic sewage is a purposeful subject. In this article, an originality tactics of anaerobic starvation for 100 days was adopted for rapidly achieving PN in actual wastewater, the nitrite accumulation rate (NAR) improved from 4.95% to 81.73% in 18 days. After anaerobic starvation was stopped, the stable PN effect furnished enough stroma for the growth of anammox bacteria. The abundance of Candidatus Brocadia grew from 0% to 0.42% in floc sludge and 0.43% in blank biofilm, which promoted nitrogen removal effect. Anaerobic starvation continuing 74 days generated further decrease in the abundance of Nitrobacter and Nitrospira of nitriteoxidizing bacteria (NOB), indicating that anaerobic starvation can restore the destroyed partial nitrification. In conclusion, this article furnished a low-cost method for achieving anammox bacteria self-enrichment in mainstream municipal wastewater in 10% filling ratio without chemicals addition.
Keyword ：
Partial nitrification Partial nitrification Anammox bacteria self -enrichment Anammox bacteria self -enrichment Anaerobic starvation Anaerobic starvation Municipal wastewater Municipal wastewater
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| GB/T 7714 | Han, Xueke , Zhang, Liyuan , Yuan, Yue et al. Anaerobic starvation realizes partial nitrification and starts anammox bacteria self-enrichment in mainstream municipal sewage treatment in a low filling ratio sequencing batch reactor [J]. | BIORESOURCE TECHNOLOGY , 2023 , 387 . |
| MLA | Han, Xueke et al. "Anaerobic starvation realizes partial nitrification and starts anammox bacteria self-enrichment in mainstream municipal sewage treatment in a low filling ratio sequencing batch reactor" . | BIORESOURCE TECHNOLOGY 387 (2023) . |
| APA | Han, Xueke , Zhang, Liyuan , Yuan, Yue , Zhang, Qiong , Peng, Yongzhen . Anaerobic starvation realizes partial nitrification and starts anammox bacteria self-enrichment in mainstream municipal sewage treatment in a low filling ratio sequencing batch reactor . | BIORESOURCE TECHNOLOGY , 2023 , 387 . |
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