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学者姓名：王秀红
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Abstract ：
Intracellular probing at single cell resolution is key to revealing the heterogeneity of cells, learning new cell subtypes and functions, understanding the pathophysiology of disease, and ensuring precise diagnosis and treatment. Despite the best efforts, an enormous challenge remains due to the very small size, extremely low content, and dynamic microenvironment of a single cell. Whispering gallery mode (WGM) micro/nanolasers (active WGM) offer unique advantages of small mode volume, high quality factors, bright and low threshold laser emission, and narrow line width, particularly suitable for integration within a single cell. In this review, we provide a focused overview of WGM micro/nanolasers for intracellular probing. We deliver information on WGM micro/nanolaser concepts, sensing mechanism, and biocompatibility, as well as recent progress in intracellular probing applications mainly covering cellular-level sensing, molecular-level detection, and feasibility for cellular imaging. At the end, challenges and prospects of WGM micro/nanolasers for intracellular applications are discussed.
Keyword ：
Sensitivity enhancement Sensitivity enhancement Intracellular molecular quantification Intracellular molecular quantification Cell tracking Cell tracking Single cell resolution Single cell resolution Deep tissue imaging Deep tissue imaging Cellular heterogeneity Cellular heterogeneity Cell tagging Cell tagging Dynamic monitoring Dynamic monitoring WGM micro/nanolaser WGM micro/nanolaser Intracellular sensing Intracellular sensing
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| GB/T 7714 | Fu, Yiqian , Lin, Siqi , Wang, Xiu-Hong . Whispering Gallery Mode Micro/Nanolasers for Intracellular Probing at Single Cell Resolution [J]. | ACS SENSORS , 2024 , 9 (11) : 5683-5698 . |
| MLA | Fu, Yiqian 等. "Whispering Gallery Mode Micro/Nanolasers for Intracellular Probing at Single Cell Resolution" . | ACS SENSORS 9 . 11 (2024) : 5683-5698 . |
| APA | Fu, Yiqian , Lin, Siqi , Wang, Xiu-Hong . Whispering Gallery Mode Micro/Nanolasers for Intracellular Probing at Single Cell Resolution . | ACS SENSORS , 2024 , 9 (11) , 5683-5698 . |
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Abstract ：
Exosome-based liquid biopsies highlight potential utility in diagnosis and determining the prognosis of patients with cancer and other diseases. However, the existing techniques are severely limited for practical applications due to the complications of high cost, low sensitivity, tedious procedures, and large sample consumption. Herein, we report a microstructured optical fiber sensor for fast, sensitive, and accurate quantification of exosomes in blood samples of breast cancer patients. Numerical simulations are applied to demonstrate that hollow-core microstructured anti-resonant fibers (HARFs) can stringently confine light in the fiber core, ensuring strong light-matter interaction and thus maximumly amplifying the signal. Taking this advantage, a AuNPs-dsDNA assembly containing gold nanoparticles, a recognizing DNA aptamer, and a fluorescent reporter DNA sequence is fabricated followed by immobilization on the fiber wall to form a AuNPs- dsDNA-HARF sensor. Cancer-derived exosomes can be recognized and captured in the fiber channel and generate dose-dependent fluorescent signals for quantification. The microfiber sensor demonstrates enhanced sensitivity and specificity, enabling the detection of single digits of exosome particles at the nanoliter sample level. In addition, by tracking exosome phenotypic changes, the proposed fiber sensor can facilitate precise drug treatment monitoring. This work provides a robust platform for exosome-based biopsy for cancer diagnosis and prediction of therapeutic outcomes.
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| GB/T 7714 | Liu, Zihao , Zhang, Wen , Zhang, Xin et al. Microstructured Optical Fiber-Enhanced Light-Matter Interaction Enables Highly Sensitive Exosome-Based Liquid Biopsy of Breast Cancer [J]. | ANALYTICAL CHEMISTRY , 2023 , 95 (2) : 1095-1105 . |
| MLA | Liu, Zihao et al. "Microstructured Optical Fiber-Enhanced Light-Matter Interaction Enables Highly Sensitive Exosome-Based Liquid Biopsy of Breast Cancer" . | ANALYTICAL CHEMISTRY 95 . 2 (2023) : 1095-1105 . |
| APA | Liu, Zihao , Zhang, Wen , Zhang, Xin , Wang, Shijia , Xia, Zhiwen , Guo, Xiaoyan et al. Microstructured Optical Fiber-Enhanced Light-Matter Interaction Enables Highly Sensitive Exosome-Based Liquid Biopsy of Breast Cancer . | ANALYTICAL CHEMISTRY , 2023 , 95 (2) , 1095-1105 . |
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Abstract ：
Microstructured optical fibers (MOFs) provide solutions for breaking through the bottlenecks in areas of high-power transmission and high-efficiency optical waveguides. Other than transporting light waves, MOFs can synergistically combine microfluidics and optics in a single fiber with an unprecedented light path length not readily achievable by planar optofluidic configurations. Here, we demonstrate that hollow-core anti-resonant optical fibers (HcARFs) can significantly enhance Raman scattering by over three orders of magnitude (EF approximate to 5000) compared with a planar setup, due to the joint mechanisms of strong light-matter interaction in the fiber core and the cumulative effect of the fiber. The giant enhancement enables us to develop the first optical fiber sensor to achieve single cancer exosome detection via a sandwich-structured strategy. This enables a multiplexed analysis of surface proteins of exosome samples, potentially allowing an accurate identification of the cellular origin of exosomes for cancer diagnosis. Our findings could expand the applications of HcARF in many exciting areas beyond the waveguide.
Keyword ：
hollow-core anti-resonant optical fiber hollow-core anti-resonant optical fiber cancer diagnosis cancer diagnosis single exosome detection single exosome detection strong light-matter interaction strong light-matter interaction enhancement of Raman scattering enhancement of Raman scattering
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| GB/T 7714 | Xia, Zhiwen , Zhang, Xin , Yao, Jingyuan et al. Giant Enhancement of Raman Scattering by a Hollow-Core Microstructured Optical Fiber Allows Single Exosome Probing [J]. | ACS SENSORS , 2023 , 8 (4) : 1799-1809 . |
| MLA | Xia, Zhiwen et al. "Giant Enhancement of Raman Scattering by a Hollow-Core Microstructured Optical Fiber Allows Single Exosome Probing" . | ACS SENSORS 8 . 4 (2023) : 1799-1809 . |
| APA | Xia, Zhiwen , Zhang, Xin , Yao, Jingyuan , Liu, Zihao , Jin, Yulong , Yin, Huabing et al. Giant Enhancement of Raman Scattering by a Hollow-Core Microstructured Optical Fiber Allows Single Exosome Probing . | ACS SENSORS , 2023 , 8 (4) , 1799-1809 . |
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Abstract ：
The authors introduce an active-mode optical microcavity sensor with enhanced sensitivity via Forster resonant energy transfer. Changes in lasing wavelengths of both donor and acceptor enable quantitative molecular analysis and real-time monitoring of intracellular molecules. Single cell analysis is crucial for elucidating cellular diversity and heterogeneity as well as for medical diagnostics operating at the ultimate detection limit. Although superbly sensitive biosensors have been developed using the strongly enhanced evanescent fields provided by optical microcavities, real-time quantification of intracellular molecules remains challenging due to the extreme low quantity and limitations of the current techniques. Here, we introduce an active-mode optical microcavity sensing stage with enhanced sensitivity that operates via Forster resonant energy transferring (FRET) mechanism. The mutual effects of optical microcavity and FRET greatly enhances the sensing performance by four orders of magnitude compared to pure Whispering gallery mode (WGM) microcavity sensing system. We demonstrate distinct sensing mechanism of FRET-WGM from pure WGM. Predicted lasing wavelengths of both donor and acceptor by theoretical calculations are in perfect agreement with the experimental data. The proposed sensor enables quantitative molecular analysis at single cell resolution, and real-time monitoring of intracellular molecules over extended periods while maintaining the cell viability. By achieving high sensitivity at single cell level, our approach provides a path toward FRET-enhanced real-time quantitative analysis of intracellular molecules.
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| GB/T 7714 | Wang, Yaping , Lang, Marion C. , Lu, Jinsong et al. Demonstration of intracellular real-time molecular quantification via FRET-enhanced optical microcavity [J]. | NATURE COMMUNICATIONS , 2022 , 13 (1) . |
| MLA | Wang, Yaping et al. "Demonstration of intracellular real-time molecular quantification via FRET-enhanced optical microcavity" . | NATURE COMMUNICATIONS 13 . 1 (2022) . |
| APA | Wang, Yaping , Lang, Marion C. , Lu, Jinsong , Suo, Mingqian , Du, Mengcong , Hou, Yubin et al. Demonstration of intracellular real-time molecular quantification via FRET-enhanced optical microcavity . | NATURE COMMUNICATIONS , 2022 , 13 (1) . |
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Abstract ：
本发明涉及一种结合贵金属纳米颗粒的新型空芯反谐振光纤传感器及检测方法，可应用于对生化领域痕量物质的检测，属于生物医学光子学中的光纤传感领域。进行检测时，首先利用精密注射仪将目标分析物充入功能化反谐振空芯光纤(HARF)中，孵育一段时间使其与功能化界面充分反应。然后由连续激光器发射出的激光通过平凸透镜聚焦并耦合进功能化HARF内。此时由于目标分析物与其适体的特异性结合，荧光报告分子脱离并被激发出荧光。荧光恢复值与目标分析物的浓度呈线性关系。最后从HARF中散射回的光先通过滤波片滤除光源部分，再利用光谱仪收集并分析定量。
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| GB/T 7714 | 王秀红 , 刘子豪 . 一种结合贵金属纳米颗粒的新型空芯反谐振光纤传感器及检测方法 : CN202210807117.5[P]. | 2022-07-06 . |
| MLA | 王秀红 et al. "一种结合贵金属纳米颗粒的新型空芯反谐振光纤传感器及检测方法" : CN202210807117.5. | 2022-07-06 . |
| APA | 王秀红 , 刘子豪 . 一种结合贵金属纳米颗粒的新型空芯反谐振光纤传感器及检测方法 : CN202210807117.5. | 2022-07-06 . |
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本发明涉及一种基于空芯反谐振光纤和表面增强拉曼光谱的生化传感器，用于解决光纤生化传感器在癌症检测方法中低浓度灵敏度低、检测疾病单一、侵犯性强、特异性差的问题，属于生物医学光子学中光纤传感领域。本发明以修饰了特异性捕获适体的空芯反谐振光纤为捕获界面，用修饰了特异性识别适体和拉曼报告分子的拉曼探针来识别待测物，构建了新型复用光纤生化传感器。该方法利用空芯反谐振光纤增强光与物质的相互作用能力来增强拉曼信号，实现对低浓度样本的灵敏检测；该方法利用拉曼光强与待测物质之间良好的线性关系，通过检测拉曼光强的变化实现对待测物质的定量灵敏检测；利用拉曼光谱的优良复用性，通过检测系统中不同拉曼探针上对应拉曼报告分子的特征峰实现不同待测物的同时诊断。本发明可用于检测癌症外泌体、化学分子、葡萄糖、细胞、蛋白质等利用核酸适体可识别的物质，具有灵敏度高的特点，且可实现非侵入式不同疾病的同时检测。
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| GB/T 7714 | 王秀红 , 夏知文 . 一种基于空芯反谐振光纤和表面增强拉曼光谱的生化传感器 : CN202210807094.8[P]. | 2022-07-06 . |
| MLA | 王秀红 et al. "一种基于空芯反谐振光纤和表面增强拉曼光谱的生化传感器" : CN202210807094.8. | 2022-07-06 . |
| APA | 王秀红 , 夏知文 . 一种基于空芯反谐振光纤和表面增强拉曼光谱的生化传感器 : CN202210807094.8. | 2022-07-06 . |
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Abstract ：
本发明涉及一种基于FRET‑WGM微腔激光传感系统提高汞离子检测限的方法，可以提高Hg2+的测量灵敏度，应用于生化领域内金属离子的定量特异性检测。传感器的制作包括检测探针溶液的准备以及微腔传感平台的制备。进行检测时，首先利用移液枪将目标分析物加入探针溶液中，孵育一段时间使其与探针充分反应生成络合物。再加入微腔，利用静电吸附使微腔与络合物之间的距离满足FRET产生的条件。由脉冲激光器发射出的激光通过聚焦透镜聚焦并耦合进功能化微腔内。此时由于目标分析物所生成的络合物与微腔之间满足发生FRET的条件，DG微腔作为供体传递能量，供体和受体的激光峰产生。微腔谐振峰蓝移值与目标分析物的浓度呈洛伦兹线性关系，实现对样品信号的光谱检测和样品成像。
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| GB/T 7714 | 王秀红 , 王师佳 . 一种基于FRET-WGM微腔激光传感系统提高汞离子检测限的方法 : CN202211107201.2[P]. | 2022-09-09 . |
| MLA | 王秀红 et al. "一种基于FRET-WGM微腔激光传感系统提高汞离子检测限的方法" : CN202211107201.2. | 2022-09-09 . |
| APA | 王秀红 , 王师佳 . 一种基于FRET-WGM微腔激光传感系统提高汞离子检测限的方法 : CN202211107201.2. | 2022-09-09 . |
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Abstract ：
一种相变材料包裹介孔二氧化硅的纳米粒的制备方法，属于生物医学领域和纳米材料领域。相变材料的制备；将制备好的相变材料置于摇床中，调至适当PH，加入EDC和NHS活化，加入预先分散好的氨基修饰介孔二氧化硅继续摇晃2h，最后加至磷脂溶液中涡旋后冰中快速冷却，放至室温后进行离心清洗，弃上清液，将包裹好相变材料的介孔二氧化硅纳米颗粒重新分散在去离子水中。包裹了相变材料‑脂肪酸的介孔二氧化硅不仅有效地防止了介孔二氧化硅载药的泄露，而且加强了药物的精准释放，提高了药物利用率。
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| GB/T 7714 | 王秀红 , 李艳艳 . 一种相变材料包裹介孔二氧化硅的纳米粒的制备方法 : CN202110378835.0[P]. | 2021-04-08 . |
| MLA | 王秀红 et al. "一种相变材料包裹介孔二氧化硅的纳米粒的制备方法" : CN202110378835.0. | 2021-04-08 . |
| APA | 王秀红 , 李艳艳 . 一种相变材料包裹介孔二氧化硅的纳米粒的制备方法 : CN202110378835.0. | 2021-04-08 . |
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Abstract ：
In this paper, we present a novel optical microfluidic cytometry scheme for label-free detection of cells that is based on the self-mixing interferometry (SMI) technique. This device enables simple, fast and accurate detection of the individual cell characteristics and efficient cell type classification. We also propose a novel parameter to classify the cell or particle size. Artificial polystyrene beads and human living cells were measured using this system, and the SMI signal properties were statistically evaluated. The capability of the proposed cytometer for cell type discrimination and size classification has been validated by the measurement results. Our study can provide a very simple technique for cell enumeration and classification without any extra devices such as high-speed camera, photomultiplier and spectrometer. Moreover, the fluorescence staining operation which is necessary in traditional flow cytometry methods is not required either in our system.
Keyword ：
flow cytometry flow cytometry self-mixing interferometry self-mixing interferometry label-free label-free microfluidic chip microfluidic chip hydrodynamic focusing hydrodynamic focusing
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| GB/T 7714 | Zhao, Yu , Shen, Xuefei , Zhang, Menglei et al. Self-Mixing Interferometry-Based Micro Flow Cytometry System for Label-Free Cells Classification [J]. | APPLIED SCIENCES-BASEL , 2020 , 10 (2) . |
| MLA | Zhao, Yu et al. "Self-Mixing Interferometry-Based Micro Flow Cytometry System for Label-Free Cells Classification" . | APPLIED SCIENCES-BASEL 10 . 2 (2020) . |
| APA | Zhao, Yu , Shen, Xuefei , Zhang, Menglei , Yu, Jingwen , Li, Jintao , Wang, Xiuhong et al. Self-Mixing Interferometry-Based Micro Flow Cytometry System for Label-Free Cells Classification . | APPLIED SCIENCES-BASEL , 2020 , 10 (2) . |
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Abstract ：
Photoacoustic imaging, a novel biomedical imaging technique that combines the advantages of optical imaging and acoustic imaging, offers high-resolution biological tissue imaging to facilitate the observation of deeper imaging sites. In other words, it breaks the "soft limit" of conventional optical bioimaging techniques. However, many diseases, especially in the early stage, present no obvious photoacoustic contrast; therefore, it is crucial to identify effective exogenous photoacoustic contrast agents. Here we introduce a novel two-dimensional material, antimonene nanoflakes (AMNFs), which demonstrates great optical absorption from 300 nm to 900 nm as well as excellent photothermal conversion efficiency and photoacoustic performance. This material is expected to be useful as a contrast agent, helping to achieve excellent photoacoustic imaging of ultra-small tumors in vivo.
Keyword ：
biological optics biological optics two-dimensional materials two-dimensional materials tumor imaging tumor imaging contrast agent contrast agent photoacoustic imaging photoacoustic imaging
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| GB/T 7714 | Yu Jingwen , Wang Xiuhong , Feng Jinchao et al. Antimonene Nanoflakes as a Photoacoustic Imaging Contrast Agent for Tumor in vivo Imaging [J]. | CHINESE JOURNAL OF LASERS-ZHONGGUO JIGUANG , 2020 , 47 (2) . |
| MLA | Yu Jingwen et al. "Antimonene Nanoflakes as a Photoacoustic Imaging Contrast Agent for Tumor in vivo Imaging" . | CHINESE JOURNAL OF LASERS-ZHONGGUO JIGUANG 47 . 2 (2020) . |
| APA | Yu Jingwen , Wang Xiuhong , Feng Jinchao , Zhang Na , Wang Pu . Antimonene Nanoflakes as a Photoacoustic Imaging Contrast Agent for Tumor in vivo Imaging . | CHINESE JOURNAL OF LASERS-ZHONGGUO JIGUANG , 2020 , 47 (2) . |
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