A　novel　method　for　fast　determination　of　nicotine　in　hunman　plasma　and　urine　was　introduced　by　using　high　performance　liquid　chromatography　(HPLC)　coupled　with　ionic　liquid　based　liquid　phase　microextraction　(LPME)　technique　for　sample　preparation.　Some　effective　parameters　on　extraction　including　extraction　solvent,　pH　of　donor　phase,　extraction　time,　extraction　temperature　and　stirring　speed　was　investigated.　The　nicotine　enrichment　factor　was　18　under　optimum　conditions.　The　linear　range　was　0.1-50　mu　g/mL　with　a　squared　regression　coefficient　of　0.9981　and　the　detection　limit　was　0.05　mu　g/mL　(S/N=3).　The　recoveries　of　spiked　samples　range　from　91.1%　to　99.4%　with　a　relative　standard　deviation　lower　than　5.0%.