Molybdenum　disulfide　(MoS2)　is　widely　used　in　membrane　separation　because　of　its　outstanding　chemical　and　structural　stability.　In　this　study,　MoS2　nanosheets　were　in-situ　grown　on　a　tubular　ceramic　substrate　through　one-step　hydrothermal　synthesis　with　the　graphene　quantum　dots　(GQDs)　incorporated　in　the　precursor　solution.　The　growth　of　the　MoS2　nanosheets　was　inhibited　due　to　the　combination　with　GQDs　through　the　Mo-O　bond.　Therefore,　the　number　of　transport　channels　in　the　MoS2/GQDs　composite　membrane　were　increased　to　improve　the　permeability.　The　results　indicated　that　the　MoS2/GQDs　composite　membrane　have　excellent　permeances　for　both　the　aqueous　solution　and　organic　solvent.　Moreover,　the　MoS2/GQDs　composite　membrane　can　be　continuously　operated　in　cross-flow　filtration　device　for　100　h　because　the　in-situ　hydrothermal　method　has　greatly　improved　the　stability　of　membrane.　The　results　indicated　that　the　MoS2/GQDs　composite　membrane　exhibits　great　potential　in　nanofiltration.