Antibiotic　resistant　bacteria　(ARB)　and　the　antibiotic　resistance　genes　(ARGs)　dissemination　via　plasmid-mediated　conjugation　have　attracted　considerable　attentions.　In　this　research,　sulfidated　nanoscale　zerovalent　iron　(S-nZVI)/peroxymonosulfate　(PMS)　and　S-nZVI/peroxydisulfate　(PDS)　process　were　investigated　to　inactivate　ARB　(Escherichia　coli　DH5　alpha　with　RP4　plasmid,　Pseudomonas.　HLS-6　contains　sul1　and　intI1　on　genome　DNA　sequence).　S-nZVI/PMS　system　showed　higher　efficiency　than　S-nZVI/PDS　on　ARB　inactivation.　Thus,　the　optimal　condition　28　mg/L　S-nZVI　coupled　with　153.7　mg/L　(0.5　mM)　PMS　was　applied　to　remove　both　intracellular　ARGs　(iARGs)　and　ARB.　The　oxidative　damage　of　ARB　cell　was　systemically　studied　by　cell　viability,　intracellular　Mg2+　levels,　the　changes　of　extracellular　and　internal　structure,　integrity　of　cell　walls　and　membranes　and　enzymatic　activities.　S-nZVI/PMS　effectively　inactivated　ARB　(similar　to　7.32　log)　within　15　min.　These　effects　were　greatly　higher　than　those　achieved　individually.　Moreover,　removal　efficiencies　of　iARGs　sul1,　intI1　and　tetA　were　1.52,　1.79　and　1.56　log,　respectively.　These　results　revealed　that　S-nZVI　and　PMS　have　a　synergistic　effect　against　ARB　and　iARGs.　The　regrowth　assays　illustrated　that　the　ARB　were　effectively　inactivated.　By　verifying　the　inhibitory　impacts　of　S-nZVI/PMS　treatment　on　conjugation　transfer,　this　work　highlights　a　promising　alternative　technique　for　inhibiting　the　horizontal　gene　transfer.