Controlling　excessive　biomass　is　essential　for　maintaining　the　long-term　stable　operation　of　gas　biofilters.　Current　methods　based　on　physical　and　chemical　technologies　can　reduce　removal　efficiency.　In　this　study,　we　developed　a　new　method　using　enzymatic-quorum　quenching　(QQ)　to　simultaneously　control　biomass　and　maintain　a　stable　removal　efficiency.　A　70-d　biofilter　operation　showed　that　clogging　was　controlled　with　the　application　of　enzymatic-QQ,　thereby　maintaining　a　stable　operation　performance.　The　pressure　drop　of　the　biofilter　with　QQ　enzyme　(QQBF)　was　75　Pam(-　1),　while　that　of　the　control　biofilter　(BF)　was　156　Pam(-　1).　Moreover,　the　biomass　accumulation　of　QQBF　was　only　130.58　kg　m(-3),　while　that　of　BF　was　218.50　kg　m(-3).　Further　analysis　indicated　that　enzymatic-QQ　decreased　biofilm　adhesion　strength.　Accordingly,　the　biofilm　inside　the　QQBF　detached　more　easily　from　the　filler　surface　than　that　in　the　BF.　The　16S　rDNA　gene　sequencing　results　suggested　that　the　enzymatic-QQ　regulated　the　related　functional　genes,　thereby　changing　the　biofilm　characteristics.　Moreover,　the　reduced　biofilm　thickness　and　lower　inactivated　microbes　in　QQBF　enhanced　the　metabolic　activity.　Overall,　our　results　demonstrated　that　QQ　is　a　promising　technology　for　regulating　biofilm　characteristics　and　controlling　the　clogging　of　gas　biofilters.