Partial　nitrification/anaerobic　ammonium　oxidation　(anammox)　(PN/A)　was　considered　as　an　economically　beneficial　and　environmentally　friendly　biological　nitrogen　removal　process.　The　elimination　of　nitrate　produced　by　PN/A　system　was　the　key　to　improving　the　total　nitrogen　removal　efficiency　(TNRE)　of　the　system　under　mainstream　conditions.　In　this　study,　the　feasibility　of　the　coupling　S(0)-driven　autotrophic　denitrification　(SAD)　with　PN/A　in　a　single　sequencing　moving　bed　biofilm　reactor　(SMBBR)　at　room　temperature　was　explored.　Additionally,　biological　treatment　process　is　ideal　environments　for　the　emerge　and　spread　of　emerging　pol-lutants　antibiotic　resistance　genes　(ARGs),　therefore,　the　shifts　of　ARGs　in　the　system　were　investigated.　After　the　coupling　of　PN/A　and　SAD,　SMBBR　was　steadily　operated　with　high　TNRE.　Nitrite-oxidizing　bacteria　(Nitrospira　and　Candidatus　Nitrotoga)　and　nitrite　oxidoreductase　enzyme　were　inhibited　by　SAD　process.　And　anammox　bacteria　were　enriched,　especially　oligotype　GAGTTTAAT　of　Ca.　Brocadia.　Sulfurimonas　became　the　predominant　autotrophic　denitrifier.　Heterotrophic　denitrifiers　Thauera　and　Dechloromonas　were　elutriated　out　from　the　sys-tem　during　the　coupling　of　PN/A　and　SAD　process,　and　they　might　be　potential　hosts　for　ARGs,　leading　to　the　decrease　of　ARGs.　Overall,　this　study　proposed　a　new　strategy　that　SAD　could　promote　the　TNRE　of　PN/A　and　cut　the　risks　of　ARGs　propagation,　and　PN/A-SAD　coupling　system　was　a　biological　nitrogen　removal　technology　with　promising　applications　under　mainstream　conditions.