The　copper　mine　drainage　(CMD)　formed　during　copper　mining　and　smelting　has　the　characteristics　of　being　low　pH,　with　a　high　sulfate　concentration　and　large　amounts　of　metal　ions　such　as　Fe,　Cu,　and　Mn.　In　order　to　recover　copper-dominated　many　resources　from　CMD,　realize　effective　control　of　environmental　pollution　and　avoid　the　loss　of　precious　resources.　The　combined　process　of　Fe　pretreatment　recovery,　Cu　treatment　recovery,　and　S　treatment　recovery　was　established　based　on　H2O2　oxidation,　sulfate-reducing　bacteria　(SRB)　reduction,　and　sulfur-oxidizing　bacteria　oxidation.　A　pilot-scale　study　was　carried　out　with　a　CMD　inlet　flow　rate　of　12　m3/d.　Results　show　that　the　average　Cu2+　removal　rate　was　99.66%,　and　the　recovered　dry　matter　precipitated　(mainly　was　CuS)　copper　content　was　35.6%.　In　addition,　the　average　total　Fe,　Mn　and　Mg　removal　rate　was　99.46%,　99.10%　and　84.08%.　And　the　S2-oxidation　rate　was　above　90%　(mainly　converted　to　sulfur).　27.52%　Fe　precipitated　dry　matter　and　42.9%　S,　32.2%　Mg,　and　13.8%　Mn　precipitated　dry　matter　were　also　recovered　as　by-products.　Through　high-throughput　analysis　of　the　functional　and　structural　changes　in　the　SRB　community,　showed　that　indigenous　SRB　from　the　copper　mine　exhibit　good　adaptability　to　CMD　conditions,　with　the　enrichment　and　acclimation　of　SRBs　achieving　a　high　level　of　expression　of　bioactive.　The　bottleneck　of　SRB　applied　to　CMD　treatment　was　solved.　Finally,　the　economic　feasibility　analysis　shows　that　the　combined　process　treatment　CMD　can　save　58.6%　of　the　cost　compared　with　traditional　lime　neutralization　method,　and　avoid　the　sludge　treatment　cost　of　29.03　million　USD　per　year.　The　above　provides　engineering　practical　reference　for　CMD　treatment　and　resource　recovery.