It　is　of　great　significance　to　design　a　fluorescent　probe　to　monitor　glutathione　(GSH)　in　living　cells　for　disease　researches.　Herein,　we　reported　a　reactive　near-infrared　(NIR)　fluorescent　probe　N-Rh-GSH　for　the　detection　of　GSH.　The　Rhodamine　derivative　was　used　as　the　fluorescent　matrix　for　the　probe,　and　the　response　signal　was　realized　through　action　of　glutathione　(GSH)　to　trigger　the　switch　of　the　screw.　The　probe　N-Rh-GSH　showed　favourable　selectivity　and　fast　response　towards　GSH,　and　the　fluorescent　matrix　released　by　the　open　loop　exhibited　extremely　strong　fluorescence　signals　at　760　nm.　The　living　cell　imaging　assays　demonstrated　that　the　probe　N-Rh-GSH　can　successfully　track　GSH.　©　2022,　Youke　Publishing　Co.,Ltd.　All　rights　reserved.