Hydrogen　sulfide　(H2S)　has　been　found　to　be　an　important　biological　regulator　that　plays　important　roles　in　many　physiological　and　pathological　processes.　Near-infrared　(NIR)　fluorescent　probes　capable　of　selectively　detecting　H2S　in　vivo　will　be　useful　tools　to　understand　its　mechanisms　in　biological　processes.　Herein,　we　reported　an　easily　synthesized　and　stimuli-responsive　NIR　fluorescent　probe　(QCy7-HS)　for　selective　evaluation　of　endogenous　H2S　in　the　living　cells　and　mice.　In　response　to　cellular　H2S　stimulus,　QCy7-HS　is　converted　to　(tCy7　and　shows　a　unique　off-on　near-infrared　fluorescence　signal　change.　The　results　of　selectivity　and　kinetic　studies　indicated　that　our　probe　has　high　H2S　binding　capacity.　Therefore,　this　probe　was　used　for　the　fluorescence　detection　of　H2S　in　cells.　Moreover,　the　probe　was　applied　for　study　exogenous　and　endogenous　H2S　in　live　mice,　indicating　that　the　new　probe　can　be　used　as　an　efficient　tool　on　H2S　related　events　in　living　animals.　(C)　2019　Elsevier　B.V.　All　rights　reserved.