The　effect　of　organic　matters　and　nitrite　on　the　anaerobic　ammonium　oxidation　(anammox)-denitrification　coupling　process　was　studied　in　a　continuous　flow　reactor　and　batch　tests.　During　the　continuous　flow　experiment,　the　effect　of　glucose　on　the　nitrogen　removal　performance　of　the　anammox　granules　reactor　was　investigated　with　the　addition　of　sufficient　nitrite.　As　a　result,　the　granules　performed　excellent　anammox-denitrification　coupling　activities　with　the　COD　concentration　of　100mg/L.　While　the　COD　concentration　was　improved　to　200mg/L,　the　granules　performed　bad　anammox-denitrification　coupling　activities.　With　the　COD　concentrations　of　100　and　200mg/L,　the　corresponding　ammonium　removal　activities　of　granules　through　anammox　process　were　0.096　and　0.071kg　NH4+-N/(kgVSS•d).　The　nitrite　removal　activities　of　granules　through　anammox　process　were　0.153　and　0.092kg　NO2--N/(kgVSS•d).　Meanwhile,　the　corresponding　denitrification　activities　of　the　granules　were　0.111　and　0.212kg　NO2--N/(kgVSS•d).　As　for　the　batch　tests,　the　effect　of　carbon　sources　and　chemical　oxygen　demand　(COD)/NO2--N　ratios　on　the　nitrogen　removal　performance　of　the　anammox-denitrification　coupling　granules　was　analyzed.　With　the　carbon　source　of　glucose　and　the　COD/NO2--N　ranges　of　1　to　4,　the　anammox　bacteria　performed　greater　nitrite　competitive　ability　than　denitrifiers.　However,　with　the　carbon　source　of　sodium　acetate　and　the　COD/NO2--N　ranges　of　1　to　4,　the　anammox　bacteria　performed　lower　nitrite　competitive　ability　than　denitrifiers.　©　2018,　Science　Press.　All　right　reserved.