Important　biogeochemical　processes　occur　in　sediments　at　fine　scales.　Sampling　techniques　capable　of　yielding　information　with　high　resolution　are　therefore　needed　to　investigate　chemical　distributions　and　fluxes　and　to　elucidate　key　processes　affecting　chemical　fates.　In　this　study,　a　high-resolution　diffusive　gradients　in　thin-films　(DGT)　technique　was　systematically　developed　and　tested　in　a　controlled　sediment　system　to　measure　organic　contaminants,　antibiotics,　for　the　first　time.　The　DGT　probe　was　used　to　resolve　compound　distributions　at　the　mm　scale.　It　also　reflected　the　fluxes　from　the　sediment　pore-water　and　remobilization　from　the　solid　phase,　providing　more　dynamic　information.　Through　the　fine　scale　detection,　a　reduction　of　re-supply　was　observed　over　time,　which　was　concentration　and　location　dependent.　Compared　to　the　Rhizon　sampling　method,　antibiotic　concentrations　obtained　by　DGT　probes　were　less　than　the　pore-water　concentrations,　as　DGT　measures　the　labile　fraction　of　the　compounds.　The　DGT　probe　was　also　tested　on　an　intact　sediment　core　sampled　from　a　lake　in　China　and　used　to　measure　the　distribution　of　labile　antibiotics　with　depth　in　the　core　at　the　mm　scale.　Environmental　Implication:　The　abuse　of　antibiotics　and　widespread　of　their　residues　influences　the　ecosystem,　induces　the　generation　of　super-bacteria,　and　finally　poses　threat　to　human　health.　Sediments　adsorbs　pollutants　from　the　aquatic　environment,　while　may　also　release　them　back　to　the　environment.　We　systematically　developed　DGT　probe　approach　for　measuring　antibiotics　in　sediment　in　situ　in　high　resolving　power,　it　provides　information　at　fine　scale　to　help　us　investigate　biogeochemical　processes　take　place　in　sediment　and　sediment-water　interface.　©　2024　Elsevier　B.V.