Fluorescence　Resonance　Energy　Transfer　(FRET)　has　emerged　as　a　predominant,　highly　sensitive,　and　homogeneous　optical　analytical　technique　in　the　realm　of　analytical　testing　and　bio-imaging.　Gold　nanoparticles　(AuNPs)　demonstrate　a　size-dependent,　broader　absorption　range　within　visible　wavelengths　owing　to　the　phenomenon　of　surface　plasmon　resonance.　As　a　result,　they　can　effectively　act　as　light　acceptors,　enabling　the　creation　of　a　donor-acceptor　system　crucial　for　achieving　precise　target　analyte　analysis.　In　this　comprehensive　review,　we　present　an　extensive　survey　of　recent　research　advancements　in　the　field　of　FRET　techniques　based　on　AuNPs　for　the　analytical　detection　of　a　wide　range　of　entities,　including　some　biomolecules,　pesticides,　enzymes,　microorganisms,　food　safety　and　environmental　pollutants.　Additionally,　we　elucidate　the　procedural　strategies　and　underlying　mechanisms　involved.　Finally,　we　provide　perspectives　on　the　current　issues　and　future　efforts　surrounding　the　FRET　applications　of　AuNPs　in　biological　analysis.　Overall,　this　review　aims　to　provide　a　holistic　comprehension　of　gold　nanoparticle　applications　in　life　analysis　using　FRET,　while　also　presenting　a　promising　vision　for　future　endeavors　in　this　domain.　©　2024　The　Authors