Distraction　osteogenesis　(DO)　is　a　valuable　surgical　method　for　limb　lengthening　and　bone　defect　correction,　but　its　lengthy　consolidation　phase　presents　challenges.　The　accordion　technique　(AT),　involving　compression　and　distraction　of　bone　segments,　has　shown　potential　for　enhancing　healing.　This　study　aimed　to　investigate　the　effectiveness　of　the　AT　conducted　at　three　different　time　points　(distraction　phase,　early　consolidation　phase,　or　late　consolidation　phase)　compared　to　conventional　DO　in　a　mouse　osteotomy　model.　Healing　was　evaluated　using　in　vivo　microCT,　histology,　and　computational　modeling.　Results　showed　that　bridging　frequency,　BV,　and　callus　tissue　composition　were　similar　between　conventional　DO　and　late　consolidation　AT.　In　contrast,　distraction　phase　AT　led　to　delayed　healing　at　day　15　with　a　72%　reduction　in　BV　compared　to　DO,　but　no　significant　differences　by　the　endpoint.　Early　consolidation　AT　showed　significantly　impaired　healing　compared　to　DO,　with　only　29%　of　mice　achieving　bony　bridging,　and　significantly　reduced　bone　marrow　area　of　the　endpoint　callus.　In　silico　modeling　was　generally　predictive　of　in　vivo　findings　and　suggested　that　application　of　the　AT　during　early　consolidation　results　in　destruction　of　newly-formed　vascular　tissue.　Overall,　no　benefit　was　observed　for　the　AT　compared　to　conventional　DO　with　the　parameters　employed　in　this　study.