Chinese　cities　are　increasingly　developing　exclusive　bicycle　paths　to　improve　the　safety　and　efficiency　of　bicycle　transit.　The　width　of　bikeways　is　a　critical　factor　influencing　cyclists＇　safety　and　comfort,　with　insufficient　width　identified　as　a　major　contributor　to　bicycle　accidents.　Therefore,　determining　the　minimum　operational　width　for　human-powered　bicycles　is　essential　for　bikeway　design.　While　some　countries＇　design　manuals　consider　speed　as　a　factor　in　determining　width,　there　is　a　lack　of　field　experiments　to　validate　these　specifications　from　the　perspective　of　cyclists＇　safety　and　comfort.　This　study　addresses　this　gap　by　conducting　a　field　experiment　to　measure　cycling　workload,　which　reflects　safety　and　comfort　under　different　widths　and　cycling　speeds.　The　experiment　involved　12　cyclists　on　a　test　road,　where　cycling　workload　was　measured　at　various　preset　widths　and　cycling　speeds　for　a　single　human-powered　cyclist.　The　results　were　further　validated　using　conventional　lateral　distance　measurement　techniques,　which　are　used　in　the　existing　literature　to　determine　the　cycling　width.　The　results　show　that　wider　bikeway　widths　lead　to　a　lower　cycling　workload,　enhancing　comfort　and　safety.　However,　both　very　high　(over　20　km/h)　and　very　low　(under　5　km/h)　speeds　significantly　increase　cyclists＇　workload,　which　in　turn　requires　a　wider　path　to　maintain　a　safe　and　comfortable　cycling　experience.　The　study　found　that　a　minimum　width　of　0.90　m　may　be　adequate　for　cyclists　traveling　at　speeds　between　10　and　15　km/h,　while　a　width　of　1.0　m　is　sufficient　for　speeds　ranging　from　5　km/h　to　25　km/h,　provided　the　bicycle　width　does　not　exceed　0.62　m.　Given　that　cyclists　typically　progress　from　slower　to　faster　speeds,　a　minimum　operational　width　of　1.0　m　is　recommended　for　most　cases.　This　study　highlights　the　importance　of　considering　cyclists＇　workload　in　determining　appropriate　bikeway　widths.　It　provides　valuable　insights　for　designing　safer,　more　comfortable　bike　paths　and　reducing　bicycle　accidents,　contributing　to　the　sustainable　development　of　urban　cycling　infrastructure.