Study on performance of ANAMMOX reactor and the functional bacteria at room temperature - Details

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Abstract：

At　room　temperature,　by　Changing　the　substrate　concentration　of　inflow,　the　biological　nitrogen　removal　of　up-flowing　ANAMMOX　biofilter　loading　volcanic　rock　filter　at　different　ammonium　concentrations　was　investigated.　techniques　of　microscope　and　scanning　electron　microscopy　(SEM)　were　utilized　to　analyze　microorganisms　form,　denaturing　gradient　gel　electrophoresis　(DGGE),　cloning　and　sequcening　were　used　to　identify　species　of　ANAMMOX.　The　results　of　operation　revealed　that,　at　room　temperature,　the　up-flow　ANAMMOX　biofilter　had　the　ability　to　maintain　high　capacity　of　biological　nitrogen　removal　under　high　and　low　substrate　concentrations.　The　maximum　total　nitrogen　removal　load　was　up　to　2.99kgN/(m3·d),　and　the　maximum　removal　rate　of　NH4+-N　and　NO2--N　was　99.4%　and　98.8%　respectively.　Microscope　and　SEM　show　that　the　species　of　microorganisms　is　much　richer　at　the　bottom　of　biofilter,　however,　the　proportion　of　ANAMMOX　is　much　higher　in　the　middle　and　upper　of　biofilter.　The　sequence　of　ANAMMOX　16SrRNA　identification　revealed　that　Candidatus　Kuenenia　stuttgartiensis　occurred　in　the　up-flowing　ANAMMOX　biofilter,　which　had　wide　adaptability　to　different　temperature　and　substrate　concentrations.

Keyword：

Biofilters Microorganisms Scanning electron microscopy Clone cells Electrophoresis Nitrogen removal Wastewater treatment

Author Community：

[ 1 ] [Li, Dong]Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China
[ 2 ] [Qiu, Wen-Xin]Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China
[ 3 ] [Zhang, Nan]Goudian Dongbei Environ. Protection Industry Group Co., Ltd Xiannvhe Wasterwater Treatment Plant, Shenyang 110005, China
[ 4 ] [Wu, Di]Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China
[ 5 ] [Zeng, Tao-Tao]State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China
[ 6 ] [Chang, Xiao-Yan]Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China
[ 7 ] [Zeng, Hui-Ping]Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China
[ 8 ] [Zhang, Jie]Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China
[ 9 ] [Zhang, Jie]State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China