This　paper　analyses　the　characteristics　of　separating　pedestrian　flow　in　T-shaped　passage.　Pedestrians　would　wave　and　the　crowed　phenomenon　may　happen　in　the　connection　area　of　the　main　passage　and　branch　passage.　In　order　to　reduce　the　pedestrian　conflicts,　guide　sign　is　set　to　guide　the　pedestrians　and　subconscious　strength　is　introduced　to　show　the　effect　of　guide　sign.　On　this　basis,　simulation　model　of　separating　pedestrian　flow　in　T-shaped　passage　based　on　guide　sign　considering　unbalanced　flow　is　established.　On　the　platform　of　MATLAB　software,　separating　pedestrian　flow　simulation　with　the　effect　of　guide　sign　is　realized.　Simulations　indicate　that　efficiency　of　pedestrians　passing　the　passage　is　improved　with　the　effect　of　guide　sign,　suitable　position　of　guide　sign　is　obtained,　and　compared　with　the　separating　pedestrian　flow　based　on　guide　sign　without　considering　unbalanced　flow,　the　model　considering　unbalanced　flow　is　better　in　describing　pedestrian　distribution.