In　this　study,　co-metabolism　biodegradation　of　DCPs　was　investigated,　with　glucose　as　co-metabolism　substrate　and　four　DCPs　including　2,3-dichlorophenol　(2,3-DCP),　2,5-dichlorophenol　(2,5-DCP),　3,4-dichlorophenol　(3,4-DCP)　and　3,5-dichlorophenol　(3,5-DCP)　as　target　pollutants.　The　results　showed　that　glucose　could　accelerate　the　biodegradation　process　of　DCPs　due　to　the　reasons　that　glucose　could　enhance　the　microorganisms＇　adaptability　towards　DCPs　as　a　co-metabolism　substrate　in　the　activated　sludge　and　speed　up　the　acclimation　process　of　DCPs　high-efficiency　degradation　strains,　which　indirectly　improved　the　biodegradation　rate　of　DCPs　in　activated　sludge.　Through　80　days　of　acclimatization,　the　microorganisms　were　able　to　degrade　6.5　mg　L-1　2,3-DCP,　7.5　mg　L-1　2,5-DCP,　6.3　mg　L-1　3,4-DCP　and　7.2　mg　L-1　3,5-DCP　in　different　bioreactors,　respectively.　With　the　stepwise　improvement　of　glucose　feed　concentration,　the　biodegradation　rate　of　DCPs　changed　gradually.　The　glucose　influent　concentrations　of　1000　mg　L-1,　1200　mg　L-1,　1000　mg　L-1　and　1200　mg　L-1　were　selected　as　optimal　experimental　value,　respectively.　Under　these　conditions,　biodegradation　concentrations　of　2,3-DCP,　2,5-DCP,　3,4-DCP　and　3,5-DCP　with　the　initial　concentration　of　15　mg　L-1　reached　up　to　11.6　mg　L-1,　12.3　mg　L-1,　11.5　mg　L-1　and　10.6　mg　L-1,　correspondingly.　Combination　with　DCPs　structure　and　effect　of　each　DCP　on　microorganisms,　results　also　demonstrated　that　the　meta-　and　papa-substituted　CPs　were　of　greater　toxicity　than　the　ortho-substituteds＇.