Through　an　aqueous,　protein-mediated　layer-by-layer　titania　deposition　process,　we　have　fabricated　a　protamine/titania　composite　layer　on　nickel　foam.　The　coating　was　composed　of　amorphous　carbon　and　TiO2(B)/anatase　nanoparticles　and　formed　upon　organic　pyrolysis　under　a　reducing　atmosphere　(5%　H-2-Ar　mixture).　X-ray　diffraction　analyses,　Auger　electron　spectroscopy,　and　high-resolution　transmission　electron　microscopy　revealed　that　the　obtained　coatings　contained　fine　monoclinic　TiO2(B)　and　anatase　nanocrystals,　along　with　amorphous　carbon.　Moreover,　the　coating　can　be　used　as　a　binder-free　negative　electrode　material　for　lithium-ion　batteries　and　exhibits　high　reversible　capacity　and　fast　charge-discharge　properties;　a　reversible　capacity　of　245　mAh　g(-1)　was　obtained　at　a　current　density　of　50　mA　g(-1),　and　capacities　of　167　and　143　mAh　g(-1)　were　obtained　at　current　densities　of　1　and　2　A　g(-1),　respectively.