To　study　the　adhesion,　proliferation　and　expression　of　bone　marrow　mesenchymal　stem　cells　(BMSCs)　on　nano-hydroxyapatite　(Nano-HA)　bone　graft　material　after　transfection　of　adenovirus-mediated　human　bone　morphogenetic　protein-2　expression　vector　(Ad-BMP-2).　BMSCs　were　transfected　using　Ad-BMP-2.　Immunohistochemistry　and　Western　blot　were　used　to　detect　BMP-2　expression　in　transfected　cells.　After　transfection,　BMP-2　protein　was　highly　expressed　in　BMSCs;　MTT　test　assay　showed　that　the　Nano-HA　bone　graft　material　could　not　inhibit　in　vitro　proliferation　of　BMSCs.　Ad-BMP-2-transfected　BMSCs　are　well　biocompatible　with　Nano-HA　bone　graft　material,　the　transfected　cells　in　material　can　secrete　BMP-2　stably　for　a　long　time.