Mesoporous　SiO2/Fe3O4　hollow　microspheres　were　used　to　immobilize　laccase　with　a　physical　adsorption　method.　The　influence　of　some　experimental　factors,　such　as　adsorption　time,　temperature　and　pH　value　on　laccase　immobilization　were　investigated.　The　pH　stability　and　temperature　stability　of　the　immobilized　laccase　were　also　studied　under　different　conditions　through　researching　its　residual　catalytic　activity.　The　surface　area　(772　m(2)/g)　and　pore　volume　(0.83　cm(3)/g)　of　the　mesoporous　SiO2/Fe3O4　hollow　microspheres　decrease　to　390　m(2)/g　and　0.66　cm(3)/g,　respectively,　after　laccase　was　immobilized　on　the　composite　microspheres.　The　immobilization　amount　of　laccase　in　the　mesoporous　silica　in　mesoporous　SiO2/Fe3O4　microspheres　is　689　mg/g,　which　is　higher　than　that　in　MCM-41　(320　mg/g).　The　residual　catalytic　activities　of　the　immobilized　laccase　at　pH　=　3　similar　to　6　and　60　degrees　C　remains　higner　than　80%　and　60%,　respectively.　The　immobilized　laccase　keeps　a　more　stable　catalytic　activity　than　free　laccase.　The　Michaelis　constant　of　immobilized　laccase　is　1.046　mmol/L.　The　affinity　of　the　immobilized　laccase　to　its　substrate　declines　slightly　compared　to　free　laccase.　The　immobilized　laccase　also　exhibits　an　excellent　catalytic　performance　in　the　removal　of　2,4-di-chlorophenol.