The　use　of　an　active　nano-interface　designed　from　gold　nanoparticles　embedded　on　ionic　liquid　for　DNA　damage　resulted　from　formalehyde　(　HCHO)　is　reported　in　this　article.　The　active　nano-interface　was　fabricated　by　depositing　gold　nanoparticles　on　the　ionic　liquid　1-butyl-3-methylimidazolium　tetrafluroborate　([bmim][BF(4)]).　A　glassy　carbon　electrode　modified　by　this　composite　film　was　fabricated　to　immobilize　DNA　for　probing　into　the　damage　resulted　from　HCHO.　The　modifying　process　was　characterized　by　X-ray　photoelectron　spectroscopy,　atomic　force　microscopy　and　electrochemistry　involving　electrochemical　impedance　spectroscopy.　It　was　found　that　the　modified　film　performs　effectively　in　studying　the　DNA　damage　by　electrocatalytic　activity　toward　HCHO　oxidation.　(C)　2009　Elsevier　B.　V.　All　rights　reserved.