The　output　characteristics　of　the　mode-locked　thulium-doped　fiber　laser　with　MoS2　as　saturable　absorber　are　simulated　based　on　rate　equation　and　nonlinear　Schrodinger　equation.　With　small　signal　gain　efficient　as　variable,　the　effect　of　pump　power,　length　of　gain　fiber,　doping　concentration　and　other　parameters　on　output　characteristics　are　analyzed.　The　theoretical　results　show　an　optimum　doping　concentration　or　fiber　length　exists　to　obtain　maximum　gain　and　average　output　power　for　given　doped　fiber.　In　experiment　a　thulium-doped　fiber　laser　is　setup　and　the　experimental　output　characteristics　of　the　laser　are　compared　with　theoretical　counterpart,　as　a　result,　theoretical　results　are　well　in　consistence　with　experimental　results.