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Abstract:
Hydroxylamine (NH2OH) as the putative intermediate for anammox ensures the robustness of partial nitritation/anammox (PN/A) process; however, the feasible for NH2OH addition to improve the stability of PN/A process under low-strength ammonia (NH4+-N) condition need to be further investigated. In this study, the restoration and steady operation of mainstream PN/A process were investigated to treat real sewage with in situ NH2OH added in a continuous alternating anoxic/aerobic with integrated fixed-film activated sludge (A(3)-IFAS) reactor. Results showed that the deteriorated PN/A process caused by nitrate (NO3--N) built-up was rapidly restored with a distinct decrease of the NO3-- N-produced/NH4+-N-consumed ratio from 28.7% to <10.0% within 20 days, after 5 mgN/L of NH2OH was added daily into the aerobic zone of A(3)-IFAS reactor. After 230 days of operation, the average total nitrogen (TN) and phosphate (PO43--P) removal efficiencies of 80.8% and 91.5%, respectively were stably achieved, with average effluent sCOD, NH4+-N, TN and PO43--P concentrations reaching 23.1, 2.3, 7.7 and 0.4 mg/L, respectively. Microbial community characterization revealed Candidatus Brocadia (3.60% and 2.92%) and Ignavibacteriae (1.56% and 2.66%) as the dominant anammox bacteria and denitrifying bacteria, respectively, jointly attached in the biofilm_1 and biofilm_2, while Candidatus Microthrix (5.17%) dominant in floc sludge was main responsible for phosphorus removal. This study confirmed that NH2OH addition is an effective strategy for nitrite-oxidizing bacteria suppression, contributing to the in situ restoration of PN/A process and high stable mainstream nitrogen and phosphorus removal in a continuous PN/A process from real sewage. (C) 2021 Elsevier B.V. All rights reserved.
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SCIENCE OF THE TOTAL ENVIRONMENT
ISSN: 0048-9697
Year: 2021
Volume: 794
9 . 8 0 0
JCR@2022
ESI Discipline: ENVIRONMENT/ECOLOGY;
ESI HC Threshold:94
JCR Journal Grade:1
Cited Count:
WoS CC Cited Count: 31
SCOPUS Cited Count: 37
ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count:
Chinese Cited Count:
30 Days PV: 3
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