Membrane　fouling　markedly　influences　the　service　life　and　performance　of　the　membrane　during　the　using　process.　Herein,　hydrophilic　polyvinylidene　fluoride　(PVDF)　nanocomposite　(P-GO-DAA)　membranes　with　antifouling　and　anti-biofouling　characteristics　were　fabricated　by　employing　graphene　oxide　(GO)　and　different　concentrations　of　D-Tyrosine.　The　structural　properties　of　the　prepared　nanocomposite　membranes　as　well　as　pure　PVDF　membranes　were　characterized　using　FTIR,　XPS,　SEM,　AFM,　and　contact　angle　analysis.　It　was　found　that　the　introduction　of　GO　fillers　made　an　excellent　antifouling　performance　compared　to　pure　PVDF　indicated　by　the　pure　water　flux,　flux　recovery　rate,　and　rejection　rate　during　ultrafiltration　experiments　as　a　result　of　the　formation　of　the　hydrophilic　and　more　porous　membrane.　In　particular,　the　nanocomposite　membranes　showed　an　increased　flux　of　305.27　L/(m(2)center　dot　h)　and　the　rejection　of　93.40%　for　the　mixed　pollutants　solution　(including　Bull　Serum　Albumin,　Sodium　Alginate,　and　Humic　Acid).　Besides,　the　outstanding　anti-biofouling　activity　was　shown　by　the　P-GO-DAA　membrane　with　the　properties　of　D-Tyrosine　for　inhibiting　biofilm　formation　during　the　bacterial　adhesion　experiments.　Furthermore,　the　adhesion　ratio　of　bacteria　on　the　membrane　was　26.64%　of　the　P-GO-DAA　membrane　compared　to　84.22%　of　pure　PVDF.　These　results　were　confirmed　by　CLSM.