Microviscosity　is　a　fundamental　parameter　in　the　biophysics　of　life　science　and　governs　numerous　cellular　processes.　Thus,　the　development　of　real-time　quantitative　monitoring　of　microviscosity　inside　cells　is　important.　The　traditional　probes　for　detecting　microviscosity　via　time-resolved　luminescence　imaging　(TRLI)　are　generally　disturbed　by　autofluorescence　or　surrounding　oxygen　in　cells.　Herein,　we　developed　loose　packing　nanoaggregates　with　aggregation-induced　delayed　fluorescence　(FKP-POA　and　FKP-PTA)　and　free　from　the　effect　of　oxygen　and　autofluorescence　for　viscosity　mapping　via　TRLI.　The　feasibility　of　FKP-PTA　nanoparticles　(NPs)　for　microviscosity　mapping　through　TRLI　was　demonstrated　by　monitoring　the　variation　of　microviscosity　inside　HepG2　cancer　cells,　which　demonstrated　a　value　change　from　14.9　cP　to　216.9　cP　during　the　apoptosis.　This　indicates　that　FKP-PTA　NP　can　be　used　as　a　probe　for　cellular　microviscosity　mapping　to　help　people　to　understand　the　physiologically　dynamic　microenvironment.　The　present　results　are　expected　to　promote　the　advancement　of　diagnostic　and　therapeutic　methods　to　cope　with　related　diseases.