The　ubiquitous　and　ineffective　treatment　of　antibiotics　in　aquatic　systems　leads　to　serious　environmental　problems,　threatening　ecology　and　human　health.　Herein,　layered　double　metal　hydroxides　(LDH)　and　biochar　(BC)　were　successfully　combined　through　a　facile　hydrothermal　method　to　prepare　a　novel　pH-universal　electric　Fenton　(EF)　cathode　(MnFe-LDH@BC).　The　developed　MnFe-LDH@BC-EF　system　exhibited　superior　performance　in　the　degradation　of　tetracycline　(TC)　(94.2%)　than　both　of　MnFe-LDH-EF　(64.1%)　and　BC-EF　system　(67.0%)　and　showed　a　well-pleasing　performance　in　a　wide　pH　range.　It　was　proposed　that　the　nanostructures　of　LDH　favored　the　multivalent　cycle　of　Fe　and　Mn,　promoting　the　electron　transfer　of　BC　as　well　as　the　release　of　Fe2+,　leading　to　continuously　generation　of　extensive　free　radicals　and　non-radicals.　The　co-existing　ions　(Cl-,　NO3-,　CO32-,　HCO3-　and　H2PO4-)　had　different　impact　on　the　removal　of　TC.　The　studied　system　exhibited　satisfactory　degradation　of　various　selected　contaminants　as　well　as　fantastic　stability　in　successive　cycles.　The　possible　degradation　pathways　were　proposed　and　the　toxicity　of　intermediate　products　were　assessed　by　the　Estimation　Program　Interface　(EPI)　Suite　analysis.　This　work　demonstrated　the　great　potential　of　the　developed　cathode　in　water　treatment　area　and　provided　insights　into　the　utilization　of　biomass　waste　resources.　©　2022　Elsevier　Ltd.