In　order　to　further　verify　the　feasibility　of　the　application　of　bio-oil　modified　asphalt　in　asphalt　pavement,　this　work　mixed　different　amounts　of　bio-oil　into　the　asphalt,　and　the　impact　and　mechanism　of　the　micro-technical　performance　of　bio-oil　on　asphalt　were　studied　by　means　of　molecular　dynamics　technology　and　macro　experiments.　In　this　work,　microscopic　evaluation　indicators　such　as　cohesive　energy　density　and　Hansen　solubility　were　introduced,　the　rationality　of　the　asphalt　model　was　verified　by　the　relationship　between　dynamic　modulus　and　cohesive　energy　density,　and　the　microscopic　properties　including　microscopic　Young＇s　modulus,　microscopic　Poisson＇s　ratio,　microscopic　viscosity　and　solubility　parameters　were　further　studied.　The　results　show　that　the　molecular　model　of　bio-oil　modified　asphalt　based　on　the　average　molecular　structure　of　triglyceride　(TG)　has　good　reliability;　in　micro　scale,　the　greater　the　cohesive　energy　density　of　the　asphalt　binder,　the　stronger　the　bond　between　the　molecules　in　the　asphalt　binder,　and　the　better　the　ability　to　resist　shear　deformation　macroscopically;　the　increasing　bio-oil　can　reduce　the　Young＇s　modulus　and　Poisson＇s　ratio　of　the　asphalt　model;　under　a　certain　load,　the　incorporation　of　bio-oil　can　make　the　asphalt　binder　softer,　and　the　horizontal　deformation　also　gets　smaller.　Similarly,　the　shear　modulus　of　the　asphalt　binder　model　decreases　with　the　amount　of　bio-oil.　In　addition,　the　Hansen　solubility　parameters　of　SBS　modifier,　biomass　oil　and　asphalt　were　calculated.　It　is　confirmed　that　the　microscopic　reason　for　the　poor　storage　stability　of　SBS　modified　asphalt　binder　is　the　difference　of　the　Hansen　solubility　parameters　between　SBS　and　asphalt　binder.　Furthermore,　it　is　found　that　the　Hansen　solubility　parameter　of　bio-oil　and　asphalt　binder　is　closer,　compared　with　SBS.　From　the　microscopic　mechanism,　it　can　be　inferred　that　the　storage　stability　of　bio-oil　modified　asphalt　binder　is　better　than　that　of　SBS-modified　asphalt　binder.　The　influence　of　biomass　oil　on　the　microscopic　properties　of　asphalt　has　been　proved,　and　the　scientific　method　of　studying　asphalt　materials　has　been　further　expanded.　The　storage　and　application　of　oil-modified　asphalt　in　asphalt　road　construction　provides　a　theoretical　basis.　©　2022　Cailiao　Daobaoshe/　Materials　Review.　All　rights　reserved.