Zwitterionic　nanofiltration　membranes　(ZNFMs)　are　conducive　to　achieve　efficient　antibiotic/monovalent　salt　separation.　However,　preparation　of　ZNFMs　through　interfacial　polymerization　is　limited　by　electrostatic　interaction　between　zwitterions.　In　this　study,　inorganic　salts　were　applied　to　regulate　reactions　in　interfacial　polymerization,　such　as　interfacial　reaction　and　thermal　cross-linking　reaction.　The　electrostatic　interaction　between　zwitterions　was　broken　by　the　inorganic　salts　to　promote　cross-linking　of　zwitterionic　polyamide　net-works.　Therefore,　an　intact　and　ultrathin　separation　layer　was　formed　on　substrates.　The　obtained　ZNFMs　exhibited　a　high　permeance　of　21.6　L　m-　2　h-1　bar-1,　while　the　rejections　for　erythromycin　(ERY)　and　NaCl　were　97.5%　and　8.5%,　respectively.　Moreover,　the　ZNFMs　also　had　an　exceptional　anti-fouling　property,　which　showed　a　great　potential　in　antibiotic/monovalent　salt　separation.