The　single　molecule　localization　microscopy　technology,　as　one　of　super-resolution　imaging　technique,　plays　an　extremely　important　role　in　various　fields,　such　as　biomedicine　and　3D　tracking.　Much　attention　has　been　paid　to　pursue　high　accuracy　axial　localization　and　isotropical　three-dimensional　super-resolution　microscopy.　However,　those　techniques　that　encode　the　axial　position　of　the　molecule　by　introducing　aberration　to　system　Point　Spread　Function　results　in　mutual　restriction　between　axial　resolution　and　imaging　depth.　In　contrast,　fluorescence　self-interference　digital　holography　can　encode　the　3D　position　of　a　single　molecule　naturally,　which　is　reflected　in　the　different　shapes　and　density　distribution　of　interference　fringes.　Combined　with　some　suitable　retrieve　algorithm,　the　3D　localization　of　single　molecule　can　be　achieved　without　the　problem　of　three-dimensional　spatial　resolution　anisotropy.　We　proposed　here　a　new　method　of　encoding　and　extracting　the　3D　position　of　fluorescent　micro　particles.　Cross-correlation　matching　method　is　employed　to　localize　the　axial　position　of　fluorescent　microsphere.　And　the　localization　accuracy　of　the　proposal　is　compared　with　that　of　the　traditional　diffraction　reconstruction　algorithm.　The　effectiveness　of　the　method　is　verified　by　both　simulations　and　experiments,　and　the　localization　accuracy　of　the　method　is　evaluated　quantitatively.　The　results　show　that　localization　accuracy　by　the　proposal　is　better　than　that　of　the　diffraction　propagation　algorithm.　Our　proposal　provides　an　alternative　idea　for　single　molecule　localization　imaging.　This　method　overcomes　the　limitation　of　the　imaging　depth　in　the　traditional　single　molecule　localization,　reduces　the　influence　of　optical　aberration　on　the　localization　accuracy,　and　it　possesses　the　potential　of　achieving　the　isotropic　super　resolution　localization　of　the　sample.