Single-molecule　localization　microscopy　(SMLM)　has　been　applied　as　a　powerful　tool　in　the　visualization　of　biological　samples.　The　obtained　details　about　the　fine　structures　inside　the　cells　or　tissues,　on　the　spatial　scale　of　much　smaller　than　the　size　that　defined　by　the　diffraction　limit　of　the　optical　system,　are　important　for　the　understanding　of　the　dynamics　and　organization　of　the　living　process.　In　this　paper,　by　exploring　the　direct　correlation　of　real-valued　holograms　in　fluorescence　self-interference　digital　holography　(SIDH),　an　imaging　method　is　proposed　for　3D　localization　and　dynamic　3D　tracking　of　nanoparticles.　The　trend　of　3D　localization　accuracy　under　different　axial　positions　is　studied　in　detail.　An　appropriate　mathematical　model　is　used　to　fit　the　cross-correlation　curve　of　the　hologram　to　determine　the　axis　coordinate　of　the　point　source.　The　validity　of　the　proposed　method　is　demonstrated　in　simulation　and　experiments.　This　method　does　not　require　phase　shift　technology.　The　3D　coordinates　of　particles　can　be　obtained　through　a　single　real-valued　hologram　using　the　proposed　algorithm.　Therefore,　we　believe　the　developed　method　has　unique　advantages　in　the　3D　visualization　of　dynamic　events　that　happened　on　the　biomolecules　inside　the　cellular　structures.　©　2024　Elsevier　Ltd