Since　the　breakthrough　discovery　of　N6-methyladenosine　(m6A),　the　field　of　RNA　epitranscriptomics　has　attracted　increasing　interest　in　the　biological　sciences.　Transfer　RNAs　(tRNAs)　are　extensively　modified,　and　various　modifications　play　a　crucial　role　in　the　formation　and　stability　of　tRNA,　which　is　universally　required　for　accurate　and　efficient　functioning　of　tRNA.　Abnormal　tRNA　modification　can　lead　to　tRNA　degradation　or　specific　cleavage　of　tRNA　into　fragmented　derivatives,　thus　affecting　the　translation　process　and　frequently　accompanying　a　variety　of　human　diseases.　Increasing　evidence　suggests　that　tRNA　modification　pathways　are　also　misregulated　in　human　cancers.　In　this　review,　we　summarize　tRNA　modifications　and　their　biological　functions,　describe　the　type　and　frequency　of　tRNA　modification　alterations　in　cancer,　and　highlight　variations　in　tRNA-modifying　enzymes　and　the　multiple　functions　that　they　regulate　in　different　types　of　cancers.　Furthermore,　the　current　implications　and　the　potential　role　of　tRNA　modifications　in　the　progression　of　pancreatic　cancer　are　discussed.　Collectively,　this　review　describes　recent　advances　in　tRNA　modification　in　cancers　and　its　potential　significance　in　pancreatic　cancer.　Further　study　of　the　mechanism　of　tRNA　modifications　in　pancreatic　cancer　may　provide　possibilities　for　therapies　targeting　enzymes　responsible　for　regulating　tRNA　modifications　in　pancreatic　cancer.