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Author:

Wang Yaping (Wang Yaping.) | Wang Xiuhong (Wang Xiuhong.) (Scholars:王秀红) | Wang Pu (Wang Pu.)

Abstract:

Whispering gallery mode is a type of optical mode where photons move in a quasi-two-dimensional plane, and the total reflection occurs at the boundary of the microcavity without reflecting out of the cavity. This mode has a high Q value and small mode volume, and it is extremely sensitive to changes in the surrounding environment. A broadband fluorescence can be transformed into narrow-spectrum laser output by using the whispering gallery mode. In this paper, polystyrene microspheres doped with the dragon green fluorescent dye arc used as whispering gallery mode optical microcavity. Through the phagocytosis of cells, the fluorescent microspheres reach inside cells and then arc pumped by nanosecond pulsed laser to achieve the output of whispering gallery mode laser in cells. In comparison with the laser output in the pure-water environment, a redshift of the intracellular fluorescent microsphere whispering gallery mode resonance emission can be observed, and the redshift is related to cell type; therefore, it can be used for unlabeled identification of cell type.

Keyword:

biotechnology wavelength shift endocytosis microsphere cavity biosensing whispering gallery mode

Author Community:

  • [ 1 ] [Wang Yaping]Beijing Univ Technol, Beijing Engn Res Ctr Laser Technol, Key Lab Transscale Laser Mfg Technol, Inst Laser Engn,Minist Educ China, Beijing 100124, Peoples R China
  • [ 2 ] [Wang Xiuhong]Beijing Univ Technol, Beijing Engn Res Ctr Laser Technol, Key Lab Transscale Laser Mfg Technol, Inst Laser Engn,Minist Educ China, Beijing 100124, Peoples R China
  • [ 3 ] [Wang Pu]Beijing Univ Technol, Beijing Engn Res Ctr Laser Technol, Key Lab Transscale Laser Mfg Technol, Inst Laser Engn,Minist Educ China, Beijing 100124, Peoples R China

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Source :

CHINESE JOURNAL OF LASERS-ZHONGGUO JIGUANG

ISSN: 0258-7025

Year: 2020

Issue: 2

Volume: 47

Cited Count:

WoS CC Cited Count: 0

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 3

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