In　order　to　optimize　the　bus　departure　time　considering　uncertain　factors,　this　paper　constructed　an　uncertain　bi-level　programming　model　for　departure　frequency　and　scheduling　of　a　bus　line.　The　uncertainty　of　passenger　arrival　and　bus　operation　time　were　taken　into　account,　combined　with　actual　operation　conditions.　Nanchang　207　bus　line　was　taken　as　an　example　to　optimize　the　departure　frequency　and　scheduling　in　the　morning　peak　hour.　The　optimal　departure　frequency　in　the　morning　peak　hour　is　12　times.　The　overall　index　value　of　the　route＇s　non-uniform　scheduling　during　peak　hours　increased　by　0.06　and　9.23%　compared　with　uniform　scheduling.　The　analysis　results　show　that　the　effect　of　the　non-uniform　scheduling　is　obvious.　The　issue　of　bus　line　departure　frequency　and　scheduling　has　a　positive　effect　on　improving　the　efficiency　of　public　transportation,　reducing　operating　costs　and　promoting　the　sustainable　development　of　the　public　transportation　system.　This　paper　provides　a　theoretical　support　for　bus　operators　to　optimize　route　operations.