Antibiotics　are　recognized　as　effective　medicine　that　has　been　extensively　used　in　human　and　veterinary.　Since　the　rate　of　releasing　into　the　environment　is　stronger　than　the　rate　of　elimination,　antibiotics　are　regarded　as　persistent　or　“pseudo-persistent”　organic　compounds　that　result　in　the　development　of　microbial　antibiotic　resistance.　Therefore,　assessment　for　their　ecological　risks　to　the　environment　are　essential.　Diffusive　gradients　in　thin　films　for　organic　compounds　(o-DGT)　have　been　adapted　to　investigate　the　environmental　behaviors　of　antibiotics.　Currently,　more　than　20　compounds　have　been　tested　by　o-DGT　in　waters　and　soil　environments.　In　this　review,　we　explained　the　theoretical　reason　that　o-DGT　is　feasible　to　determine　the　labile　fraction　of　antibiotics　in　different　environmental　media.　The　most　used　agarose　diffusive　gel,　and　various　binding　agents　such　as　resin,　porous　carbon　and　nano-scale　materials　have　been　compared　to　optimize　the　sampling　of　antibiotics　by　o-DGT.　Results　of　deploying　o-DGT　devices　in　waters　and　soils　from　previous　studies　were　discussed　to　understand　the　bioavailability　and　dynamic　transport　of　antibiotics.　Also,　we　provided　the　feasibility　analysis　of　using　o-DGT　in　sediments　for　antibiotics　measurements,　which　is　required　to　be　carried　out　in　future　studies.　To　have　a　deep　view　on　the　development　of　o-DGT,　its　technical　limitations　and　viable　improvements　were　summarized　in　this　study　for　further　applications　on　antibiotics　research.　©　2023