The　fatigue　safety　of　cable-girder　anchorage　structures　in　cable-stayed　bridges　under　long-term　service　has　attracted　much　attention.　For　bridges　located　in　seasonally　cold　regions,　the　effect　of　low-temperature　environments　should　be　considered　when　evaluating　fatigue　performance.　Using　the　Heilongjiang　Bridge　in　China　as　a　case　study,　a　room-temperature　fatigue　test　with　a　numerical　simulation　that　considers　the　low-temperature　effect　on　both　load　effect　and　fatigue　resistance　was　proposed.　A　fatigue　test　with　increased　testing　load　amplitude　was　performed　on　a　1:3.75　ratio　specimen.　After　3.2　million　loading　cycles　and　using　an　acoustic　emission　technique,　no　fatigue　crack　was　observed　in　the　anchorage　structure.　The　extended　finite　element　method　was　then　adopted　to　analyze　the　anchorage　zone’s　fatigue　crack　initiation　position　and　propagation　path.　Finally,　based　on　the　fatigue　characteristics　of　bridge　steel,　the　fatigue　resistance　to　the　crack　propagation　of　the　vulnerable　area　was　evaluated　under　three　different　service　conditions.　The　results　show　that　the　fatigue　performance　of　the　anchorage　zone　at　low　temperatures　is　sufficient.　Moreover,　this　paper　provides　a　more　widely　applicable　and　cost-effective　approach　for　the　fatigue　evaluation　of　steel　bridges.　©　2023　by　the　authors.