Detection of Cancer Cells Based on Fluorescence Quenching Property of Black Phosphorus - Details

Author：

Yan, Wujuan (Yan, Wujuan.) | Wang, Xiuhong (Wang, Xiuhong.) (Scholars：王秀红) | Yao, Qian (Yao, Qian.) | Qiao, Pengfei (Qiao, Pengfei.) | Lang, Marion C (Lang, Marion C.)

Indexed by：

EI Scopus PKU CSCD

Abstract：

Black　phosphorus　(BP)　nanosheets　can　quench　the　fluorescence　of　a　fluorescent　dye,　and　based　on　this　property,　the　deoxyribonucleic　acid(DNA)　aptamer　which　can　specifically　recognize　the　human　breast　cancer　cells　MCF7　are　synthesized　and　labeled　with　5-carboxyfluorescein　(FAM).　When　the　DNA　aptamer　is　mixed　with　BP　nanosheets,　its　fluorescence　is　quenched.　However,　in　the　presence　of　MCF7　cells,　the　DNA　aptamer　can　recognize　the　MCF7　cells　and　interact　with　these　cells,　and　the　disassociation　of　DNA　aptamer　from　BP　nanosheets　occurs　and　then　the　restoration　of　fluorescence　is　realized.　The　fluorescence　intensity　shows　the　linear　relationship　with　the　number　of　cancer　cells.　The　experimental　results　indicate　that　it　is　highly　efficient,　specific　and　sensitive　to　use　BP　nanosheets　for　detecting　breast　cancer　cells　and　this　method　can　be　applied　in　the　detection　of　other　kinds　of　cancer　cells.　©　2018,　Chinese　Lasers　Press.　All　right　reserved.

Keyword：

Cytology Fluorescence Phosphorus Diseases Nanosheets Black Phosphorus Cells Fluorescence quenching Quenching DNA

Author Community：

[ 1 ] [Yan, Wujuan]Laboratory for Biomedical Photonics, Institute of Laser Engineering, Beijing University of Technology, Beijing; 100011, China
[ 2 ] [Wang, Xiuhong]Laboratory for Biomedical Photonics, Institute of Laser Engineering, Beijing University of Technology, Beijing; 100011, China
[ 3 ] [Yao, Qian]Laboratory for Biomedical Photonics, Institute of Laser Engineering, Beijing University of Technology, Beijing; 100011, China
[ 4 ] [Qiao, Pengfei]Laboratory for Biomedical Photonics, Institute of Laser Engineering, Beijing University of Technology, Beijing; 100011, China
[ 5 ] [Lang, Marion C]Laboratory for Biomedical Photonics, Institute of Laser Engineering, Beijing University of Technology, Beijing; 100011, China