【Objective】Endophytic　bacteria　with　good　resistance　to　Phytophthora　were　screened　from　cucumber　leaves　and　identified,　so　as　to　provide　experimental　materials　for　further　study　of　theoretical　exploration　and　their　application　in　controlling　cucumber　blight.【Method】Endophytic　bacteria　were　screened　from　cucumber　leaves,　and　were　preliminarily　screened　according　to　microscopic　characteristics,　Gram　staining　reaction,　colony　arrangement　and　colony　morphology.　The　antagonism　of　endophytic　bacteria　was　tested　by　plate　antagonism　method　and　fermentation　liquor　antagonism　method.　Through　16S　rDNA　sequencing　and　homology　analysis,　phylogenetic　tree　was　constructed　to　identify　the　target　strains.　【Result】Thirty-two　endophytic　bacteria　were　preliminarily　screened　and　numbered　as　CL1-CL32,　including　10　strains　of　Gram-negative　bacillus,　13　strains　of　Gram-positive　bacillus　and　9　strains　of　Gram-positive　cocci.　Eight　strains　were　found　to　have　strong　inhibitory　effect　on　Phytophthora　drechsleri　by　plate　confrontation　method,　and　the　inhibition　rate　of　mycelia　ranged　from　10.3%　to　54.3%.　The　inhibition　radius　ranged　from　1　mm　to　7　mm.　Three　strains　(CL7,　CL9,　CL15)　with　better　inhibitory　effect　were　tested　in　fermentation　liquor　antagonism　experiment,　and　the　inhibition　rates　were　54.4%,　72.2%,　63.3%,　respectively.【Conclusion】The　three　endophytic　bacteria　(CL7,CL9,CL15)　with　strong　inhibitory　effect　on　P.　drechsleri　belonged　to　Pantoea　sp.,　Pseudomonas　sp.,and　Staphylococcus　sp.,　among　which　CL9　belonging　to　Pseudomonas　sp.　had　the　most　obvious　inhibitory　effect.　©　2022　Guangdong　Agricultural　Sciences.　All　rights　reserved.