Fungi　with　multiple　contaminant　removal　function　have　rarely　been　studied.　Here,　a　novel　fungal　strain　Fusarium　keratoplasticum　FSP1,　which　was　isolated　from　halophilic　granular　sludge,　is　reported　for　first　time　to　perform　simultaneous　nitrogen　and　phosphate　removal.　The　strain　showed　wide　adaptability　under　C/N　ratios　of　30–35,　salinities　of　0　%–3　%　(m/v),　and　pH　of　7.5–9.5.　The　maximum　removal　rates　of　ammonium,　nitrate　and　nitrite　were　4.43,　4.01　and　2.97　mg　N/L/h.　The　nitrogen　balance,　enzyme　activity　and　substrate　conversion　experiments　demonstrated　a　single　strain　FSP1　can　assimilate　inorganic　nitrogen　and　convert　inorganic　nitrogen　to　gaseous　nitrogen　through　heterotrophic　nitrification　or　aerobic　denitrification.　About　39　%–42　%　of　the　degraded　phosphorus　was　in　the　extracellular　polymeric　substances　(EPS).　Orthophosphate　was　the　main　phosphorus　species　in　the　cell,　whereas　phosphate　monoester　and　diester　were　in　the　EPS.　The　novel　strain　FSP1　is　a　potential　candidate　for　wastewater　treatment.　©　2023　Elsevier　Ltd