Living　single-cell　analysis　is　of　great　significance　for　revealing　cell　heterogeneity,　understanding　biological　processes　and　mechanisms,　and　preventing　and　early　diagnosing　major　diseases.　Intracellular　delivery　is　a　key　step　in　gene　editing,　drug　delivery　and　immunotherapy,　etc.　At　present,　there　are　many　intracellular　delivery　methods,　most　of　which　are　based　on　population　cells　and　can　only　achieve　a　single　direction　of　delivery.　Moreover,　the　delivery　efficiency　is　limited,　and　the　activity　of　most　cells　is　affected　using　these　methods.　However,　the　intracellular　delivery　methods　of　living　single　cells　based　on　the　nanotip　structure　can　pass　through　the　cell　membrane　and　establish　channels　inside　and　outside　the　membrane.　In　the　process　of　cargos　delivery,　the　activity　of　the　single　cells　is　basically　not　affected,　and　the　bidirectional　delivery　can　be　achieved　with　a　high　cell　utilization　rate.　What　is　more,　single-cell　localization　and　quantitative　intracellular　delivery　can　be　realized.　In　order　to　comprehensively　understand　the　current　situation　of　intracellular　delivery　of　living　single　cells　based　on　nanotip　structures　and　promote　the　research　and　development　of　intracellular　delivery　methods,　the　research　progresses　of　intracellular　delivery　methods　of　living　single　cells　based　on　nanotip　structures　in　recent　years　were　reviewed　in　this　paper.　According　to　whether　the　nanotip　structure　is　hollow　or　not,　it　can　be　divided　into　two　categories:　solid　nanotips　and　hollow　nanotips.　The　materials,　sizes　and　preparation　methods　of　nanotip　were　summarized.　The　driving　force,　principle,　advantages　and　disadvantages　of　intracellular　delivery　methods　based　on　nanotip　structures　were　discussed.　Finally,　the　development　trend　of　these　methods　based　on　nanotip　structures　was　prospected.