Modern　chromatography　is　increasingly　focused　on　miniaturization　and　integration.　Compared　to　conventional　liquid　chromatography,　microfluidic　chip　liquid　chromatography　(microchip-LC)　has　the　potential　due　to　its　zero-dead　volume　connection　and　ease　of　integration.　Nano-sized　packings　have　the　potential　to　significantly　enhance　separation　performance　in　microchip-LC.　However,　their　application　has　been　hindered　by　packing　difficulties.　This　study　presents　a　method　for　packing　nano-sized　silica　particles　into　a　microchannel　as　the　stationary　phase.　The　microchip-LC　packed　column　was　prepared　by　combining　the　weir　and　the　porous　silica　single-particle　as　frit　to　retain　the　packing　particles.　A　surface　tension-based　single-particle　picking　technique　was　established　to　insert　porous　single-particle　frit　into　glass　microchannels.　Additionally,　we　developed　a　slurry　packing　method　that　utilizes　air　pressure　to　inject　nano-sized　packing　into　the　microchannel.　Pressure-driven　chromatographic　separation　was　performed　using　this　nano-packed　column　integrated　into　a　glass　microchip.　The　mixture　of　four　PAHs　was　successfully　separated　within　just　8　min　using　a　5　mm　separation　channel　length,　achieving　high　theoretical　plates　(106　plates/m).　Overall,　these　findings　demonstrate　the　potential　of　utilizing　nano-sized　packings　for　enhancing　chromatographic　performance　in　microchip　systems.　©　2024