Mineralized　collagen　scaffold　is　one　of　the　best　choices　for　bone　defects　treatment,　but　weak　mechanical　strength　is　the　main　factor　restricting　its　development.　Recent　studies　demonstrated　that　despite　being　a　fundamental　form　of　mechanical　stimulation　in　human　activities,　the　impact　of　cyclic　compressive　stress　on　collagen　mineralization　remains　unclear,　with　even　less　known　about　the　dynamic　mechanical　mechanism.　This　study　employed　cyclic　compressive　stress　to　investigate　its　effect　on　collagen　mineralization.　The　findings　revealed　that　cyclic　compressive　strain　promotes　collagen　mineralization　by　facilitating　increased　mineral　penetration　into　the　collagen　and　altering　mineral　morphology　on　the　collagen　surface.　As　the　mineral　volume　fraction　of　mineralized　collagen　rises,　its　elastic　modulus　also　increases.　Additionally,　the　finite　element　simulation　results　proved　that　cyclic　compressive　stress　can　impact　mineral　distribution　by　affecting　their　transport　and　deposition,　consequently　influencing　the　stress　distribution　and　regulating　mechanical　properties　of　mineralized　collagen.　Alterations　in　mechanical　properties　provide　feedback　on　internal　stress　distribution,　subsequently　impacting　mineral　mineralization.　This　study　achieves　a　closed-loop　study　on　the　mechanical　regulated　collagen　mineralization,　offers　insight　into　the　mechanism　of　collagen　mineralization,　paving　the　way　for　further　exploration　of　biomineralization　mechanisms　and　potentially　inspiring　novel　approaches　for　the　fabrication　of　mineralized　collagen　scaffolds.　©　2024　The　Authors