The　cross-linking　process　of　collagen　is　one　of　the　more　important　ways　to　improve　the　mineralization　ability　of　collagen.　However,　the　regulatory　effect　of　dynamic　cross-linking　on　biomineralization　in　vitro　remains　unclear.　Dynamic-cross-linked　mineralized　collagen　under　different　cross-linking　processes,　according　to　the　process　of　cross-linking　and　mineralization　of　natural　bone,　was　prepared　in　this　study.　Mineralization　was　performed　for　12　h　at　4,　8,　and　12　h　of　collagen　cross-linking.　Scanning　electron　microscopy　(SEM)　and　transmission　electron　microscopy　(TEM)　showed　the　characteristics　of　dynamic-cross-linked　mineralization　in　terms　of　morphological　transformation　and　distribution.　Fourier　transform　infrared　spectroscopy　(FTIR)　analysis　showed　the　crystallinity　characteristics　of　the　hydroxyapatite　(HA)　crystal　formation.　Pre-cross-linked　dynamic-cross-linked　mineralization　refers　to　the　process　of　cross-linking　for　a　period　of　time　and　then　side　cross-linked　mineralization.　The　mineral　content,　enzyme　stability,　and　mechanical　properties　of　mineralized　collagen　were　improved　through　a　dynamic　cross-linking　process　of　pre-cross-linking.　The　swelling　performance　was　reduced　through　the　dynamic　cross-linking　process　of　pre-cross-linking.　This　study　suggests　that　the　dynamic　cross-linking　process　through　pre-cross-linking　could　make　it　easier　for　minerals　to　permeate　and　deposit　between　collagen　fibers,　improve　mineralization　efficiency,　and,　thus,　enhance　the　mechanical　strength　of　biomineralization.　This　study　can　provide　new　ideas　and　a　theoretical　basis　for　designing　mineralized　collagen　scaffolds　with　better　bone　repair　ability.