The　proliferation　of　antibiotic　resistant　genes　(ARGs)　and　antibiotic　resistant　bacteria　(ARB)　caused　by　antibiotic　abuse　has　raised　concerns　about　the　global　infectious-disease　crisis.　This　study　employed　periodate　(PI)/ferrate　(VI)　(Fe　(VI))　system　to　disinfect　Gram-negative　ARB　(Escherichia　coli　DH5α)　and　Gram-positive　bacteria　(Bacillus　subtilis　ATCC6633).　The　PI/Fe　(VI)　system　could　inactivate　1　×　108　CFU/mL　of　Gram-negative　ARB　and　Gram-positive　bacteria　by　4.0　and　2.8　log　in　30　min.　Neutral　and　acidic　pH,　increase　of　PI　dosage　and　Fe　(VI)　dosage　had　positive　impacts　on　the　inactivation　efficiency　of　ARB,　while　alkaline　solution　and　the　coexistence　of　10　mM　Cl-,　NO3-,　SO42-　and　20　mg/L　humic　acid　had　slightly　negative　impacts.　The　reactive　species　generated　by　PI/Fe　(VI)　system　could　disrupt　the　integrity　of　cell　membrane　and　wall,　leading　to　oxidative　stress　and　lipid　peroxidation.　Intracellular　hereditary　substance,　including　DNA　and　ARGs　(tetA),　would　leak　into　the　external　environment　through　damaged　cells　and　be　degraded.　The　electron　spin　resonance　analysis　and　quenching　experiments　indicated　that　Fe　(IV)/Fe　(V)　played　a　leading　role　in　disinfection.　Meanwhile,　PI/Fe　(VI)　system　also　had　an　efficient　removal　effect　on　sulfadiazine,　which　was　expected　to　inhibit　the　ARGs　transmission　from　the　source.　©　2024　Elsevier　B.V.