Single-Cell Imaging of mRNA by Target RNA-Initiated RCA - Details

Author：

Ren, X. (Ren, X..) | Wu, Y. (Wu, Y..) | Deng, R. (Deng, R..) | Li, J. (Li, J..)

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Abstract：

We　present　a　powerful　method　for　direct　mRNA　detection　based　on　ligation-based　recognition　and　in　situ　amplification,　capable　of　single-cell　imaging　mRNA　at　single-nucleotide　and　single-molecule　resolution.　Attributed　to　the　use　of　Splint　R　ligase　that　can　ligate　padlock　probe　with　RNA　as　target　template,　this　method　can　efficiently　detect　mRNA　in　the　absence　of　reverse　transcription.　This　method　enables　spatial　localization　and　correlation　analysis　of　gene　expression　in　single　cells,　which　helps　us　to　elucidate　gene　function　and　regulatory　mechanisms.　©　2024.　The　Author(s),　under　exclusive　license　to　Springer　Science+Business　Media,　LLC,　part　of　Springer　Nature.

Keyword：

Single molecule Single-cell imaging Rolling circle amplification In situ imaging

Author Community：

[ 1 ] [Ren X.]Department of Chemistry, College of Chemistry and Life Science, Beijing University of Technology, Beijing, China
[ 2 ] [Wu Y.]Department of Chemistry, College of Chemistry and Life Science, Beijing University of Technology, Beijing, China
[ 3 ] [Deng R.]College of Biomass Science and Engineering, Healthy Food Evaluation Research Center, Sichuan University, Chengdu, China
[ 4 ] [Li J.]Beijing Life Science Academy, Beijing, China
[ 5 ] [Li J.]Department of Chemistry, Center for BioAnalytical Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing, China
[ 6 ] [Li J.]New Cornerstone Science Laboratory, Shenzhen, China
[ 7 ] [Li J.]Center for BioAnalytical Chemistry, Hefei National Laboratory of Physical Science at Microscale, University of Science and Technology of China, Hefei, China

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Source ：

Methods in molecular biology (Clifton, N.J.)

ISSN： 1940-6029

Year： 2024

Volume： 2822

Page： 65-75

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ESI Highly Cited Papers on the List： 0 Unfold All

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30 Days PV： 5

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