The　digital　holography　is　characterized　by　its　full　field　of　view,　non-contact,　non-destructive,　real-time　and　quantitative　detection,　which　is　especially　suitable　for　the　three-dimensional　reconstruction　and　fast　tracking　of　living　cells　without　staining　or　labeling.　The　digital　holography　imaging　has　been　developed　rapidly　in　the　field　of　the　biomedical　science.　The　basic　principle　of　digital　holography　is　introduced　for　the　cell　detection,　and　the　major　progress　of　the　research　and　application　is　reviewed　based　on　the　cell　morphology,　biological　parameters,　physiological　status,　drug　effect　and　dynamic　analysis.　The　potential　application　and　future　research　are　discussed,　and　the　challenge　and　problems　of　digital　holography　technology　are　pointed　out.