Rapid　and　reliable　detection　method　for　African　swine　fever　virus　(ASFV)　is　proposed　by　surface-enhanced　Raman　spectroscopy　(SERS).　The　ASFV　target　DNA　can　be　specifically　captured　by　sandwich　hybridization　between　nanomagnetic　beads　and　a　SERS　probe.　Experimental　results　show　that　the　significant　Raman　signal　of　the　SERS　probe　with　gold　nanoparticles　and　a　molecular　reporter　DTNB　(5,5　＇-dimercapto-bis　(2-nitrobenzoic　acid))　can　be　adopted　for　detecting　the　hybridization　chain　reaction　of　ASFV　DNA.　The　advantage　of　the　SERS　sandwich　hybridization　assay　is　the　large　response　range　from　the　single　molecule　level　to　108　copies　per　mL,　which　not　only　can　overcome　the　tedious　time　required　for　the　amplification　reaction　but　also　provides　a　comparative　method　to　polymerase　chain　reaction.　Furthermore,　real　samples　of　African　swine　fever　virus　were　detected　from　different　subjects　of　swine　fever　virus　including　porcine　reproductive　respiratory　syndrome　virus　and　Japanese　encephalitis　virus.　The　proposed　biosensor　method　can　rapidly　detect　ASFV　correctly　within　15　min　as　a　simple,　convenient,　low-cost　detection　approach.　The　biosensor　can　be　used　as　a　platform　for　the　determination　in　biological,　food,　and　environmental　analytical　fields.