The　current　sewage　treatment　systems　are　typically　unable　to　efficiently　remove　both　nitrogen　and　phosphorus　simultaneously,　often　compromising　biological　phosphorus　removal　to　ensure　effective　nitrogen　removal.　To　resolve　this　issue,　this　study　establishes　an　independent　enhanced　biological　phosphorus　removal　(EBPR)　system　based　on　an　immobilized　fillers　nitrogen　removal　system　for　treating　actual　rural　sewage.　The　research　focuses　on　the　phosphorus　removal　performance,　operational　conditions,　and　characteristics　of　the　independent　EBPR　system.　When　fluctuations　occur　in　the　effluent　phosphorus　concentration,　phosphorus-accumulating　organisms　(PAOs)　utilize　only　29　%　of　the　available　carbon　sources,　with　the　remaining　carbon　being　fully　consumed　by　glycogen-accumulating　organisms　(GAOs).　The　presence　of　GAOs　and　low　dissolved　oxygen　(DO)　concentrations　leads　to　deteriorating　phosphorus　removal　performance　in　the　system.　To　address　this,　reducing　the　carbon　load　and　increasing　aeration　are　necessary　to　maintain　effluent　phosphorus　concentrations　below　0.5　mg　&　sdot;L-　1.　Tetrasphaera　and　Acinetobacter　are　identified　as　key　microbial　genera　responsible　for　phosphate　removal　in　this　system.　Finally,　fluorescence　excitation　emission　matrix　parallel　factor　analysis　(EEM-PARAFAC)　showed　that　fermentation　microorganisms,　represented　by　Tetrasphaera,　played　a　key　role　in　the　removal　of　tryptophan　and　tyrosine　produced　by　raw　wastewater　and　microbial　metabolism.