Metal-organic　frameworks　(MOFs)　are　widely　used　in　electro-Fenton　systems　due　to　their　large　surface　areas　and　superior　conductivities.　However,　conventional　MOFs　with　regular　structures　often　suffer　from　limited　active　sites　on　their　surfaces,　constraining　their　catalytic　performances.　To　address　this　limitation,　through　the　competitive　action　of　citric　acid　and　2-aminoterephthalic　acid　on　graphite　felt　(GF),　a　novel　defect-engineered　MIL-53　framework　(D-MIL-53(Fe/Ce)/400@GF)　was　synthesized.　We　successfully　prepared　D-MIL-53(Fe/Ce)　powder　by　adding　a　small　amount　of　citric　acid　during　the　solvothermal　synthesis　of　MIL-53(Fe/Ce).　After　calcination　under　N2　environment,　the　material　was　loaded　onto　GF　via　drip　coating,　resulting　in　the　final　preparation　of　D-MIL-53(Fe/Ce)/400@GF.　This　process　introduced　defect　structures　into　MIL-53,　significantly　enhancing　its　catalytic　properties.　Raman　spectroscopy　confirmed　a　substantial　increase　in　surface　defects,　with　the　ID/IG　ratio　rising　from　0.68　(MIL-53(Fe/Ce)/400@GF)　to　1.99　(D-MIL-53(Fe/Ce)/400@GF).　In　electro-Fenton　system,　H2O2　generation　by　D-MIL-53(Fe/Ce)/400@GF　was　2.02-fold　greater　than　that　of　MIL-53(Fe/Ce)/400@GF.　More　importantly,　the　degradation　of　amoxicillin　pollutant　by　D-MIL-53(Fe/Ce)/400@GF　catalyst　resulted　in　a　2.78-fold　increased　reaction　rate　when　compared　to　MIL-53(Fe/Ce)/400@GF.　Therefore,　active　sites　provided　by　defect　structures　could　effectively　enhance　cathodic　catalytic　performance.　A　degradation　pathway　of　amoxicillin　was　proposed　based　on　high-performance　liquid　chromatography-mass　spectrometry　combined　with　density　functional　theory　results,　demonstrating　significantly　reduced　toxicity　of　degradation　products.　Overall,　the　proposed　defective　MOF　composites　have　great　potential　for　use　in　electro-Fenton　technology　for　antibiotics　and　other　pollutants　decontamination.　©　2025　Elsevier　B.V.